| RRC ID |
64574
|
| 著者 |
Okahashi N, Okinaga T, Sakurai A, Terao Y, Nakata M, Nakashima K, Shintani S, Kawabata S, Ooshima T, Nishihara T.
|
| タイトル |
Streptococcus sanguinis induces foam cell formation and cell death of macrophages in association with production of reactive oxygen species.
|
| ジャーナル |
FEMS Microbiol Lett
|
| Abstract |
Streptococcus sanguinis, a normal inhabitant of the human oral cavity, is a common streptococcal species implicated in infective endocarditis. Herein, we investigated the effects of infection with S. sanguinis on foam cell formation and cell death of macrophages. Infection with S. sanguinis stimulated foam cell formation of THP-1, a human macrophage cell line. At a multiplicity of infection >100, S. sanguinis-induced cell death of the macrophages. Viable bacterial infection was required to trigger cell death because heat-inactivated S. sanguinis did not induce cell death. The production of cytokines interleukin-1β and tumor necrosis factor-α from macrophages was also stimulated during bacterial infection. Inhibition of the production of reactive oxygen species (ROS) resulted in reduced cell death, suggesting an association of ROS with cell death. Furthermore, S. sanguinis-induced cell death appeared to be independent of activation of inflammasomes, because cleavage of procaspase-1 was not evident in infected macrophages.
|
| 巻・号 |
323(2)
|
| ページ |
164-70
|
| 公開日 |
2011-10-1
|
| DOI |
10.1111/j.1574-6968.2011.02375.x
|
| PMID |
22092716
|
| MeSH |
Cell Death*
Cell Line
Foam Cells*
Humans
Interleukin-1beta / metabolism
Macrophages / immunology*
Macrophages / microbiology*
Reactive Oxygen Species / metabolism
Reactive Oxygen Species / toxicity*
Streptococcus / immunology
Streptococcus / pathogenicity*
Tumor Necrosis Factor-alpha / metabolism
|
| IF |
1.987
|
| リソース情報 |
| ヒト・動物細胞 |
THP-1(RCB1189) |
