Reference - Detail
RRC ID | 64627 |
---|---|
Author | Nakase I, Noguchi K, Fujii I, Futaki S. |
Title | Vectorization of biomacromolecules into cells using extracellular vesicles with enhanced internalization induced by macropinocytosis. |
Journal | Sci Rep |
Abstract |
Extracellular vesicles (EVs, exosomes) are approximately 30- to 200-nm-long vesicles that have received increased attention due to their role in cell-to-cell communication. Although EVs are highly anticipated to be a next-generation intracellular delivery tool because of their pharmaceutical advantages, including non-immunogenicity, their cellular uptake efficacy is low because of the repulsion of EVs and negatively charged cell membranes and size limitations in endocytosis. Here, we demonstrate a methodology for achieving enhanced cellular EV uptake using arginine-rich cell-penetrating peptides (CPPs) to induce active macropinocytosis. The induction of macropinocytosis via a simple modification to the exosomal membrane using stearylated octaarginine, which is a representative CPP, significantly enhanced the cellular EV uptake efficacy. Consequently, effective EV-based intracellular delivery of an artificially encapsulated ribosome-inactivating protein, saporin, in EVs was attained. |
Volume | 6 |
Pages | 34937 |
Published | 2016-10-17 |
DOI | 10.1038/srep34937 |
PII | srep34937 |
PMID | 27748399 |
PMC | PMC5066177 |
MeSH | Animals Arginine / chemistry CHO Cells Cell Count Cell Line, Tumor Cell Membrane / metabolism Cell-Penetrating Peptides / metabolism Cricetinae Cricetulus Endocytosis Exosomes / metabolism* Extracellular Vesicles / metabolism Fluorescent Dyes / chemistry HeLa Cells Humans Macromolecular Substances* Microscopy, Electron, Transmission Oligopeptides / chemistry Peptides / chemistry Pinocytosis* Ribosome Inactivating Proteins, Type 1 / chemistry* Ribosomes / chemistry* Saporins Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
IF | 3.998 |
Resource | |
Human and Animal Cells | HeLa |