RRC ID 64633
著者 Maeda K, Yasui H, Yamamori T, Matsuura T, Takao S, Suzuki M, Matsuda A, Inanami O, Shirato H.
タイトル A Nucleoside Anticancer Drug, 1-(3-C-Ethynyl-β-D-Ribo-Pentofuranosyl)Cytosine, Induces Depth-Dependent Enhancement of Tumor Cell Death in Spread-Out Bragg Peak (SOBP) of Proton Beam.
ジャーナル PLoS One
Abstract The effect of 1-(3-C-ethynyl-β-D-ribo-pentofuranosyl)cytosine (ECyd) on proton-induced cell death was evaluated in human lung carcinoma cell line A549 and Chinese hamster fibroblast cell line V79 to enhance relative biological effectiveness (RBE) within the spread-out Bragg peak (SOBP) of proton beams. Treatment with ECyd significantly enhanced the proton-induced loss of clonogenicity and increased senescence at the center, but not at the distal edge of SOBP. The p53-binding protein 1 foci formation assay showed that ECyd decelerated the rate of DNA double-strand break (DSB) repair at the center, but not the distal region of SOBP, suggesting that the ECyd-induced enhancement of proton-induced cell death is partially associated with the inhibition of DSB repair. This study demonstrated that ECyd enhances proton-induced cell killing at all positions of SOBP, except for the distal region and minimizes the site-dependent differences in RBE within SOBP. Thus, ECyd is a unique radiosensitizer for proton therapy that may be useful because it levels the biological dose within SOBP, which improves tumor control and reduces the risk of adverse effects at the distal edge of SOBP.
巻・号 11(11)
ページ e0166848
公開日 2016-1-1
DOI 10.1371/journal.pone.0166848
PII PONE-D-16-19203
PMID 27875573
PMC PMC5119790
MeSH A549 Cells Animals Antineoplastic Agents / pharmacology* Cell Death Cricetinae Cricetulus Cytosine* / analogs & derivatives Cytosine* / pharmacology DNA Breaks, Double-Stranded / drug effects DNA Breaks, Double-Stranded / radiation effects DNA Repair / drug effects DNA Repair / radiation effects Dose-Response Relationship, Drug Humans Lung Neoplasms / metabolism Lung Neoplasms / pathology Lung Neoplasms / therapy* Proton Therapy* Radiation-Sensitizing Agents / pharmacology* Tumor Suppressor Protein p53 / metabolism
IF 2.74
リソース情報
ヒト・動物細胞 V79(RCB0008) A549(RCB0098)