RRC ID 64664
著者 Vizcardo R, Rafiqul Islam SM, Maeda T, Tamaoki N, Good ML, Klemen ND, Bosch-Marce M, Jia L, Kruhlak MJ, Restifo NP.
タイトル A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants.
ジャーナル J Vis Exp
Abstract The inheritance of pre-rearranged T cell receptors (TCRs) and their epigenetic rejuvenation make induced pluripotent stem cell (iPSC)-derived T cells a promising source for adoptive T cell therapy (ACT). However, classical in vitro methods for producing regenerated T cells from iPSC result in either innate-like or terminally differentiated T cells, which are phenotypically and functionally distinct from naïve T cells. Recently, a novel three-dimensional (3D) thymic culture system was developed to generate a homogenous subset of CD8αβ+ antigen-specific T cells with a naïve T cell-like functional phenotype, including the capacity for proliferation, memory formation, and tumor suppression in vivo. This protocol avoids aberrant developmental fates, allowing for the generation of clinically relevant iPSC-derived T cells, designated as iPSC-derived thymic emigrants (iTE), while also providing a potent tool to elucidate the subsequent functions necessary for T cell maturation after thymic selection.
巻・号 (150)
公開日 2019-8-9
DOI 10.3791/58672
PMID 31449236
PMC PMC8191389
MeSH Animals Antigens, Neoplasm / immunology* Cell Culture Techniques / methods* Cell Differentiation Cell Line, Tumor Induced Pluripotent Stem Cells / cytology* Mice T-Lymphocytes / cytology T-Lymphocytes / immunology Thymus Gland / cytology* Thymus Gland / immunology*
IF 1.163
リソース情報
ヒト・動物細胞 OP9/N-DLL1(RCB2927)