| RRC ID |
64664
|
| Author |
Vizcardo R, Rafiqul Islam SM, Maeda T, Tamaoki N, Good ML, Klemen ND, Bosch-Marce M, Jia L, Kruhlak MJ, Restifo NP.
|
| Title |
A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants.
|
| Journal |
J Vis Exp
|
| Abstract |
The inheritance of pre-rearranged T cell receptors (TCRs) and their epigenetic rejuvenation make induced pluripotent stem cell (iPSC)-derived T cells a promising source for adoptive T cell therapy (ACT). However, classical in vitro methods for producing regenerated T cells from iPSC result in either innate-like or terminally differentiated T cells, which are phenotypically and functionally distinct from naïve T cells. Recently, a novel three-dimensional (3D) thymic culture system was developed to generate a homogenous subset of CD8αβ+ antigen-specific T cells with a naïve T cell-like functional phenotype, including the capacity for proliferation, memory formation, and tumor suppression in vivo. This protocol avoids aberrant developmental fates, allowing for the generation of clinically relevant iPSC-derived T cells, designated as iPSC-derived thymic emigrants (iTE), while also providing a potent tool to elucidate the subsequent functions necessary for T cell maturation after thymic selection.
|
| Volume |
(150)
|
| Published |
2019-8-9
|
| DOI |
10.3791/58672
|
| PMID |
31449236
|
| PMC |
PMC8191389
|
| MeSH |
Animals
Antigens, Neoplasm / immunology*
Cell Culture Techniques / methods*
Cell Differentiation
Cell Line, Tumor
Induced Pluripotent Stem Cells / cytology*
Mice
T-Lymphocytes / cytology
T-Lymphocytes / immunology
Thymus Gland / cytology*
Thymus Gland / immunology*
|
| IF |
1.163
|
| Resource |
| Human and Animal Cells |
OP9/N-DLL1(RCB2927) |
