論文 - 詳細
| RRC ID | 64664 |
|---|---|
| 著者 | Vizcardo R, Rafiqul Islam SM, Maeda T, Tamaoki N, Good ML, Klemen ND, Bosch-Marce M, Jia L, Kruhlak MJ, Restifo NP. |
| タイトル | A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants. |
| ジャーナル | J Vis Exp |
| Abstract |
The inheritance of pre-rearranged T cell receptors (TCRs) and their epigenetic rejuvenation make induced pluripotent stem cell (iPSC)-derived T cells a promising source for adoptive T cell therapy (ACT). However, classical in vitro methods for producing regenerated T cells from iPSC result in either innate-like or terminally differentiated T cells, which are phenotypically and functionally distinct from naïve T cells. Recently, a novel three-dimensional (3D) thymic culture system was developed to generate a homogenous subset of CD8αβ+ antigen-specific T cells with a naïve T cell-like functional phenotype, including the capacity for proliferation, memory formation, and tumor suppression in vivo. This protocol avoids aberrant developmental fates, allowing for the generation of clinically relevant iPSC-derived T cells, designated as iPSC-derived thymic emigrants (iTE), while also providing a potent tool to elucidate the subsequent functions necessary for T cell maturation after thymic selection. |
| 巻・号 | (150) |
| 公開日 | 2019-8-9 |
| DOI | 10.3791/58672 |
| PMID | 31449236 |
| PMC | PMC8191389 |
| MeSH | Animals Antigens, Neoplasm / immunology* Cell Culture Techniques / methods* Cell Differentiation Cell Line, Tumor Induced Pluripotent Stem Cells / cytology* Mice T-Lymphocytes / cytology T-Lymphocytes / immunology Thymus Gland / cytology* Thymus Gland / immunology* |
| IF | 1.163 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | OP9/N-DLL1(RCB2927) |