RRC ID |
64679
|
著者 |
Haque M, Xiong X, Lei F, Das JK, Song J.
|
タイトル |
An optimized protocol for the generation of HBV viral antigen-specific T lymphocytes from pluripotent stem cells.
|
ジャーナル |
STAR Protoc
|
Abstract |
In T cell-based cancer immunotherapy, tumor antigen (Ag)-specific CD8+ cytotoxic T lymphocytes (CTLs) can specifically target tumor Ags on malignant cells. This promising approach drove us to adopt this strategy of T cell transfer (ACT)-based immunotherapy for chronic viral infections. Here, we describe the generation of hepatitis B virus (HBV) Ag-specific CTLs from induced pluripotent stem cells (iPSCs), i.e., iPSC-CTLs. Ag-specific iPSC-CTLs can target HBV Ag+ cells and infiltrate into the liver to suppress HBV replication in a murine model. For complete details on the use and execution of this protocol, please refer to Haque et al. (2020).
|
巻・号 |
2(1)
|
ページ |
100264
|
公開日 |
2021-3-19
|
DOI |
10.1016/j.xpro.2020.100264
|
PII |
S2666-1667(20)30251-3
|
PMID |
33490980
|
PMC |
PMC7806515
|
MeSH |
Animals
Hepatitis B / immunology
Hepatitis B / therapy
Hepatitis B Antigens / immunology*
Hepatitis B virus / immunology*
Humans
Immunotherapy, Adoptive
Induced Pluripotent Stem Cells / immunology*
Mice
T-Lymphocytes, Cytotoxic / immunology*
|
リソース情報 |
ヒト・動物細胞 |
iPS-MEF-Ng-20D-17(APS0001) |