RRC ID 64679
著者 Haque M, Xiong X, Lei F, Das JK, Song J.
タイトル An optimized protocol for the generation of HBV viral antigen-specific T lymphocytes from pluripotent stem cells.
ジャーナル STAR Protoc
Abstract In T cell-based cancer immunotherapy, tumor antigen (Ag)-specific CD8+ cytotoxic T lymphocytes (CTLs) can specifically target tumor Ags on malignant cells. This promising approach drove us to adopt this strategy of T cell transfer (ACT)-based immunotherapy for chronic viral infections. Here, we describe the generation of hepatitis B virus (HBV) Ag-specific CTLs from induced pluripotent stem cells (iPSCs), i.e., iPSC-CTLs. Ag-specific iPSC-CTLs can target HBV Ag+ cells and infiltrate into the liver to suppress HBV replication in a murine model. For complete details on the use and execution of this protocol, please refer to Haque et al. (2020).
巻・号 2(1)
ページ 100264
公開日 2021-3-19
DOI 10.1016/j.xpro.2020.100264
PII S2666-1667(20)30251-3
PMID 33490980
PMC PMC7806515
MeSH Animals Hepatitis B / immunology Hepatitis B / therapy Hepatitis B Antigens / immunology* Hepatitis B virus / immunology* Humans Immunotherapy, Adoptive Induced Pluripotent Stem Cells / immunology* Mice T-Lymphocytes, Cytotoxic / immunology*
リソース情報
ヒト・動物細胞 iPS-MEF-Ng-20D-17(APS0001)