論文 - 詳細
| RRC ID | 64683 |
|---|---|
| 著者 | Suzuki H, Kasai K, Kimura Y, Miyata S. |
| タイトル | UV/ozone surface modification combined with atmospheric pressure plasma irradiation for cell culture plastics to improve pluripotent stem cell culture. |
| ジャーナル | Mater Sci Eng C Mater Biol Appl |
| Abstract |
Culturing pluripotent stem cells effectively requires substrates coated with feeder cell layers or cell-adhesive matrices. It is difficult to employ pluripotent stem cells as resources for regenerative medicine due to risks of culture system contamination by animal-derived factors, or the large costs associated with the use of adhesive matrices. To enable a coating-free culture system, we focused on UV/ozone surface modification and atmospheric pressure plasma treatment for polystyrene substrates, to improve adhesion and proliferation of pluripotent stem cells. In this study, to develop a feeder- and matrix coating-free culture system for embryonic stem cells (ESCs), mouse ESCs were cultured on polystyrene substrates that were surface-modified using UV/ozone-plasma combined treatment. mESCs could be successfully cultured under feeder-free conditions upon UV/ozone-plasma combined treatment of culture substrates, without any further chemical treatments, and showed similar proliferation rates to those of cells grown on the feeder cell layer or matrix-coated substrates. |
| 巻・号 | 123 |
| ページ | 112012 |
| 公開日 | 2021-4-1 |
| DOI | 10.1016/j.msec.2021.112012 |
| PII | S0928-4931(21)00151-X |
| PMID | 33812631 |
| MeSH | Animals Atmospheric Pressure Cell Culture Techniques Cell Differentiation Cell Proliferation Feeder Cells Mice Ozone* Plastics Pluripotent Stem Cells* |
| IF | 5.88 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | EB3(AES0139) |