RRC ID 64683
著者 Suzuki H, Kasai K, Kimura Y, Miyata S.
タイトル UV/ozone surface modification combined with atmospheric pressure plasma irradiation for cell culture plastics to improve pluripotent stem cell culture.
ジャーナル Mater Sci Eng C Mater Biol Appl
Abstract Culturing pluripotent stem cells effectively requires substrates coated with feeder cell layers or cell-adhesive matrices. It is difficult to employ pluripotent stem cells as resources for regenerative medicine due to risks of culture system contamination by animal-derived factors, or the large costs associated with the use of adhesive matrices. To enable a coating-free culture system, we focused on UV/ozone surface modification and atmospheric pressure plasma treatment for polystyrene substrates, to improve adhesion and proliferation of pluripotent stem cells. In this study, to develop a feeder- and matrix coating-free culture system for embryonic stem cells (ESCs), mouse ESCs were cultured on polystyrene substrates that were surface-modified using UV/ozone-plasma combined treatment. mESCs could be successfully cultured under feeder-free conditions upon UV/ozone-plasma combined treatment of culture substrates, without any further chemical treatments, and showed similar proliferation rates to those of cells grown on the feeder cell layer or matrix-coated substrates.
巻・号 123
ページ 112012
公開日 2021-4-1
DOI 10.1016/j.msec.2021.112012
PII S0928-4931(21)00151-X
PMID 33812631
MeSH Animals Atmospheric Pressure Cell Culture Techniques Cell Differentiation Cell Proliferation Feeder Cells Mice Ozone* Plastics Pluripotent Stem Cells*
IF 5.88
リソース情報
ヒト・動物細胞 EB3(AES0139)