| RRC ID |
64739
|
| 著者 |
Shiozaki K, Kawabe M, Karasuyama K, Kurachi T, Hayashi A, Ataka K, Iwai H, Takeno H, Hayasaka O, Kotani T, Komatsu M, Inui A.
|
| タイトル |
Neuropeptide Y deficiency induces anxiety-like behaviours in zebrafish (Danio rerio).
|
| ジャーナル |
Sci Rep
|
| Abstract |
Neuropeptide Y (NPY) controls energy homeostasis including orexigenic actions in mammalians and non-mammalians. Recently, NPY has attracted attention as a mediator of emotional behaviour and psychosomatic diseases. However, its functions are not fully understood. We established npy gene-deficient (NPY-KO) zebrafish (Danio rerio) to assess the relationship between NPY and emotional behaviours. The NPY-KO zebrafish exhibited similar growth, but pomc and avp mRNA levels in the brain were higher as compared to wild-type fish. NPY-KO zebrafish exhibited several anxiety-like behaviours, such as a decrease in social interaction in mirror test and decreased locomotion in black-white test. The acute cold stress-treated NPY-KO zebrafish exhibited anxiety-like behaviours such as remaining stationary and swimming along the side of the tank in the mirror test. Moreover, expression levels of anxiety-associated genes (orx and cck) and catecholamine production (gr, mr, th1 and th2) were significantly higher in NPY-KO zebrafish than in wild-type fish. We demonstrated that NPY-KO zebrafish have an anxiety phenotype and a stress-vulnerability like NPY-KO mice, whereby orx and/or catecholamine signalling may be involved in the mechanism actions.
|
| 巻・号 |
10(1)
|
| ページ |
5913
|
| 公開日 |
2020-4-3
|
| DOI |
10.1038/s41598-020-62699-0
|
| PII |
10.1038/s41598-020-62699-0
|
| PMID |
32246073
|
| PMC |
PMC7125123
|
| MeSH |
Animals
Animals, Genetically Modified
Anxiety / genetics*
Anxiety / physiopathology
Anxiety / psychology
Behavior, Animal / physiology
Catecholamines / metabolism
Disease Models, Animal
Energy Metabolism / physiology*
Female
Gene Knockout Techniques
Humans
Male
Neuropeptide Y / deficiency*
Neuropeptide Y / genetics
Orexins / metabolism
Receptors, Neuropeptide Y / metabolism
Signal Transduction / physiology
Stress, Psychological / physiopathology*
Zebrafish
Zebrafish Proteins / metabolism
|
| IF |
3.998
|
| リソース情報 |
| ゼブラフィッシュ |
RIKEN WT |
