RRC ID 64754
Author Hecker A, Anger P, Braaker PN, Schulze W, Schuster S.
Title High-resolution mapping of injury-site dependent functional recovery in a single axon in zebrafish.
Journal Commun Biol
Abstract In non-mammalian vertebrates, some neurons can regenerate after spinal cord injury. One of these, the giant Mauthner (M-) neuron shows a uniquely direct link to a robust survival-critical escape behavior but appears to regenerate poorly. Here we use two-photon microscopy in parallel with behavioral assays in zebrafish to show that the M-axon can regenerate very rapidly and that the recovery of functionality lags by just days. However, we also find that the site of the injury is critical: While regeneration is poor both close and far from the soma, rapid regeneration and recovery of function occurs for injuries between 10% and 50% of total axon length. Our findings show that rapid regeneration and the recovery of function can be studied at remarkable temporal resolution after targeted injury of one single M-axon and that the decision between poor and rapid regeneration can be studied in this one axon.
Volume 3(1)
Pages 307
Published 2020-6-12
DOI 10.1038/s42003-020-1034-x
PII 10.1038/s42003-020-1034-x
PMID 32533058
PMC PMC7293241
MeSH Animals Animals, Genetically Modified Axons / physiology* Larva / physiology* Larva / radiation effects Lasers / adverse effects Microscopy, Fluorescence, Multiphoton / methods* Nerve Regeneration* Recovery of Function* Spinal Cord Injuries / physiopathology* Zebrafish
IF 4.165
Zebrafish Tol-056