| RRC ID |
64754
|
| 著者 |
Hecker A, Anger P, Braaker PN, Schulze W, Schuster S.
|
| タイトル |
High-resolution mapping of injury-site dependent functional recovery in a single axon in zebrafish.
|
| ジャーナル |
Commun Biol
|
| Abstract |
In non-mammalian vertebrates, some neurons can regenerate after spinal cord injury. One of these, the giant Mauthner (M-) neuron shows a uniquely direct link to a robust survival-critical escape behavior but appears to regenerate poorly. Here we use two-photon microscopy in parallel with behavioral assays in zebrafish to show that the M-axon can regenerate very rapidly and that the recovery of functionality lags by just days. However, we also find that the site of the injury is critical: While regeneration is poor both close and far from the soma, rapid regeneration and recovery of function occurs for injuries between 10% and 50% of total axon length. Our findings show that rapid regeneration and the recovery of function can be studied at remarkable temporal resolution after targeted injury of one single M-axon and that the decision between poor and rapid regeneration can be studied in this one axon.
|
| 巻・号 |
3(1)
|
| ページ |
307
|
| 公開日 |
2020-6-12
|
| DOI |
10.1038/s42003-020-1034-x
|
| PII |
10.1038/s42003-020-1034-x
|
| PMID |
32533058
|
| PMC |
PMC7293241
|
| MeSH |
Animals
Animals, Genetically Modified
Axons / physiology*
Larva / physiology*
Larva / radiation effects
Lasers / adverse effects
Microscopy, Fluorescence, Multiphoton / methods*
Nerve Regeneration*
Recovery of Function*
Spinal Cord Injuries / physiopathology*
Zebrafish
|
| IF |
4.165
|
| リソース情報 |
| ゼブラフィッシュ |
Tol-056 |
