論文 - 詳細
| RRC ID | 64827 |
|---|---|
| 著者 | Ogoshi M, Takahashi M, Aoyagi K, Ukena K, Aizawa S, Takeuchi H, Takahashi S, Takeuchi S. |
| タイトル | Adrenomedullin 2 and 5 activate the calcitonin receptor-like receptor (clr) - Receptor activity-modifying protein 3 (ramp3) receptor complex in Xenopus tropicalis. |
| ジャーナル | Gen Comp Endocrinol |
| Abstract |
The adrenomedullin (AM) family is involved in diverse biological functions, including cardiovascular regulation and body fluid homeostasis, in multiple vertebrate lineages. The AM family consists of AM1, AM2, and AM5 in tetrapods, and the receptor for mammalian AMs has been identified as the complex of calcitonin receptor-like receptor (CLR) and receptor activity-modifying protein 2 (RAMP2) or RAMP3. However, the receptors for AM in amphibians have not been identified. In this study, we identified the cDNAs encoding calcrl (clr), ramp2, and ramp3 receptor components from the western clawed frog (Xenopus tropicalis). Messenger RNAs of amphibian clr and ramp2 were highly expressed in the heart, whereas that of ramp3 was highly expressed in the whole blood. In HEK293T cells expressing clr-ramp2, cAMP response element luciferase (CRE-Luc) reporter activity was activated by am1. In HEK293T cells expressing clr-ramp3, CRE-Luc reporter activity was increased by the treatment with am2 at the lowest dose, but with am5 and am1 at higher dose. Our results provided new insights into the roles of AM family peptides through CLR-RAMP receptor complexes in the tetrapods. |
| 巻・号 | 306 |
| ページ | 113752 |
| 公開日 | 2021-5-15 |
| DOI | 10.1016/j.ygcen.2021.113752 |
| PII | S0016-6480(21)00044-7 |
| PMID | 33711314 |
| MeSH | Adrenomedullin* / genetics Animals Calcitonin Receptor-Like Protein / genetics HEK293 Cells Humans Peptide Hormones* Receptor Activity-Modifying Protein 2 / genetics Receptor Activity-Modifying Protein 3 / genetics Receptors, Adrenomedullin / genetics Receptors, Calcitonin* / genetics Xenopus |
| IF | 2.428 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ツメガエル・イモリ | |