論文 - 詳細
| RRC ID | 64840 |
|---|---|
| 著者 | Yamamoto S, Ohta N, Matsumoto A, Horiguchi Y, Koide M, Fujino Y. |
| タイトル | Haloperidol Suppresses NF-kappaB to Inhibit Lipopolysaccharide-Induced Pro-Inflammatory Response in RAW 264 Cells. |
| ジャーナル | Med Sci Monit |
| Abstract |
BACKGROUND Haloperidol, a tranquilizing agent, is administered both to treat symptoms of psychotic disorders and to sedate agitated and delirious patients. Notably, haloperidol has been suggested to inhibit the immune response through unknown mechanisms. We hypothesized that the sedative modulates the immune response via NF-κB. MATERIAL AND METHODS Using flow cytometry, we analyzed the effects of haloperidol on expression CD80 and CD86 in RAW 264 cells and in primary macrophages derived from bone marrow. Secretion of interleukin (IL)-1β, IL-6, and IL-12 p40 was measured by enzyme-linked immunosorbent assay. In addition, NF-κB activation was evaluated using a reporter assay based on secretory embryonic alkaline phosphatase. Finally, synthetic antagonists were used to identify the dopamine receptor that mediates the effects of haloperidol. RESULTS Haloperidol inhibited NF-κB activation, and thereby suppressed expression of CD80, as well as secretion of IL-1β, IL-6, and IL-12 p40. CD80 and IL-6 levels were similarly attenuated by a D2-like receptor antagonist, but not by a D1-like receptor antagonist. CONCLUSIONS The data strongly suggest that haloperidol inhibits the immune response by suppressing NF-kB signaling via the dopamine D2 receptor. |
| 巻・号 | 22 |
| ページ | 367-72 |
| 公開日 | 2016-2-4 |
| DOI | 10.12659/msm.895739 |
| PII | 895739 |
| PMID | 26842661 |
| PMC | PMC4747317 |
| MeSH | Animals B7-1 Antigen / metabolism B7-2 Antigen / metabolism Cytokines / metabolism Female Haloperidol / pharmacology* Inflammation / metabolism Interleukins / metabolism Lipopolysaccharides / antagonists & inhibitors Lipopolysaccharides / pharmacology Macrophages / drug effects* Macrophages / metabolism Mice Mice, Inbred C57BL NF-kappa B / antagonists & inhibitors* NF-kappa B / metabolism RAW 264.7 Cells Receptors, Dopamine D2 / metabolism Schizophrenia / metabolism Signal Transduction / drug effects |
| IF | 1.918 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |