論文 - 詳細
| RRC ID | 6485 |
|---|---|
| 著者 | Shodai T, Suzuki J, Kudo S, Itoh S, Terada M, Fujita S, Shimazu H, Tsuji T. |
| タイトル | Inhibition of P-selectin-mediated cell adhesion by a sulfated derivative of sialic acid. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
P-selectin, a carbohydrate-binding cell adhesion molecule expressed on activated endothelial cells and platelets, plays a key role in the recruitment of leukocytes to inflammatory and hemorrhagic sites. It simultaneously recognizes a sialic acid-containing carbohydrate chain and the sulfated tyrosine residues of a specific counter-receptor expressed on the leukocyte surface. We examined the inhibitory effects of a synthetic sulfated derivative of sialic acid (NMSO3) on P-selectin-mediated cell adhesion and found the following: (1) P-selectin/IgG chimera bound to immobilized NMSO3. (2) The binding of P-selectin/IgG chimera to purified P-selectin glycoprotein ligand-1 was inhibited by soluble NMSO3. (3) The adhesion of HL60 cells to P-selectin-expressing CHO cells was inhibited by NMSO3. (4) NMSO3 inhibited P-selectin-induced tumor necrosis factor-alpha production in monocytes and activated platelet-induced generation of reactive oxygen species in neutrophils. In conclusion, NMSO3 acts as a specific inhibitor for P-selectin-mediated cell adhesion and for adhesion-dependent leukocyte activation. |
| 巻・号 | 312(3) |
| ページ | 787-93 |
| 公開日 | 2003-12-19 |
| DOI | 10.1016/j.bbrc.2003.10.188 |
| PII | S0006291X03023283 |
| PMID | 14680834 |
| MeSH | Animals CHO Cells Cell Adhesion / drug effects Cell Adhesion / physiology* Cricetinae Cricetulus Dose-Response Relationship, Drug HL-60 Cells Humans Leukocytes, Mononuclear Lipids / pharmacology* N-Acetylneuraminic Acid / analogs & derivatives* N-Acetylneuraminic Acid / pharmacology* Neutrophil Activation / drug effects Neutrophil Activation / physiology* Neutrophils P-Selectin / physiology* Platelet Activation / drug effects Platelet Activation / physiology Platelet Adhesiveness / drug effects Platelet Adhesiveness / physiology* Reactive Oxygen Species / metabolism Tumor Necrosis Factor-alpha / metabolism |
| IF | 2.985 |
| 引用数 | 16 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HL60(RCB001) U937 |