| RRC ID |
6485
|
| 著者 |
Shodai T, Suzuki J, Kudo S, Itoh S, Terada M, Fujita S, Shimazu H, Tsuji T.
|
| タイトル |
Inhibition of P-selectin-mediated cell adhesion by a sulfated derivative of sialic acid.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
P-selectin, a carbohydrate-binding cell adhesion molecule expressed on activated endothelial cells and platelets, plays a key role in the recruitment of leukocytes to inflammatory and hemorrhagic sites. It simultaneously recognizes a sialic acid-containing carbohydrate chain and the sulfated tyrosine residues of a specific counter-receptor expressed on the leukocyte surface. We examined the inhibitory effects of a synthetic sulfated derivative of sialic acid (NMSO3) on P-selectin-mediated cell adhesion and found the following: (1) P-selectin/IgG chimera bound to immobilized NMSO3. (2) The binding of P-selectin/IgG chimera to purified P-selectin glycoprotein ligand-1 was inhibited by soluble NMSO3. (3) The adhesion of HL60 cells to P-selectin-expressing CHO cells was inhibited by NMSO3. (4) NMSO3 inhibited P-selectin-induced tumor necrosis factor-alpha production in monocytes and activated platelet-induced generation of reactive oxygen species in neutrophils. In conclusion, NMSO3 acts as a specific inhibitor for P-selectin-mediated cell adhesion and for adhesion-dependent leukocyte activation.
|
| 巻・号 |
312(3)
|
| ページ |
787-93
|
| 公開日 |
2003-12-19
|
| DOI |
10.1016/j.bbrc.2003.10.188
|
| PII |
S0006291X03023283
|
| PMID |
14680834
|
| MeSH |
Animals
CHO Cells
Cell Adhesion / drug effects
Cell Adhesion / physiology*
Cricetinae
Cricetulus
Dose-Response Relationship, Drug
HL-60 Cells
Humans
Leukocytes, Mononuclear
Lipids / pharmacology*
N-Acetylneuraminic Acid / analogs & derivatives*
N-Acetylneuraminic Acid / pharmacology*
Neutrophil Activation / drug effects
Neutrophil Activation / physiology*
Neutrophils
P-Selectin / physiology*
Platelet Activation / drug effects
Platelet Activation / physiology
Platelet Adhesiveness / drug effects
Platelet Adhesiveness / physiology*
Reactive Oxygen Species / metabolism
Tumor Necrosis Factor-alpha / metabolism
|
| IF |
2.985
|
| 引用数 |
16
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
HL60(RCB001)
U937 |
