RRC ID 64918
著者 Sharma G, Tsutsumi K, Saito T, Asada A, Ando K, Tomomura M, Hisanaga SI.
タイトル Kinase activity of endosomal kinase LMTK1A regulates its cellular localization and interactions with cytoskeletons.
ジャーナル Genes Cells
Abstract Neurite formation, a fundamental process in neuronal maturation, requires the coordinated regulation of cytoskeletal reorganization and membrane transport. Compared to the understanding of cytoskeletal functions, less is known about the supply of membranes to growing neurites. Lemur kinase 1A (LMTK1A) is an endosomal protein kinase that is highly expressed in neurons. We recently reported that LMTK1A regulates the trafficking of Rab11-positive recycling endosomes in growing axons and dendrites. Here, we used the kinase-negative (kn) mutant to investigate the role of the kinase activity of LMTK1A in its cellular localization and interactions with the cytoskeleton in Neuro2A and PC-12 cells. Kinase activity was required for the localization of LMTK1A in the perinuclear endocytic recycling compartment. Perinuclear accumulation was microtubule dependent, and LMTK1A wild type (wt) localized mainly on microtubules, whereas kn LMTK1A was found in the actin-rich cell periphery. In the neurites of PC-12 cells, LMTK1A showed contrasting distributions depending on the kinase activity, with wt being located in the microtubule-rich shaft and the kn form in the actin-rich tip. Taken together, these results suggest that the kinase activity of LMTK1A regulates the pathway for endosomal vesicles to transfer from microtubules to actin filaments at the tip of growing neurites.
巻・号 21(10)
ページ 1080-1094
公開日 2016-10-1
DOI 10.1111/gtc.12404
PMID 27600567
MeSH Animals Apoptosis Regulatory Proteins / metabolism* Cell Line Cytoskeleton / metabolism* Endosomes / enzymology* Mice Microtubules / metabolism Neurites / metabolism* Neuronal Outgrowth PC12 Cells Protein-Tyrosine Kinases / metabolism* Rats Tubulin / metabolism
IF 1.655
リソース情報
ヒト・動物細胞 PC-12(RCB0009)