RRC ID 6493
Author Takahashi Y, Hamada J, Murakawa K, Takada M, Tada M, Nogami I, Hayashi N, Nakamori S, Monden M, Miyamoto M, Katoh H, Moriuchi T.
Title Expression profiles of 39 HOX genes in normal human adult organs and anaplastic thyroid cancer cell lines by quantitative real-time RT-PCR system.
Journal Exp Cell Res
Abstract HOX genes are well known as master control genes in embryonic morphogenesis. We hypothesized that HOX genes give cells spatial information to maintain tissue- or organ-specificity in adult body and that the deregulated expression of HOX genes results in tumor development. We established a comprehensive analysis system to quantify expression of 39 human HOX genes based on the real-time reverse transcription PCR (RT-PCR) method. Analysis of 39 HOX genes of 20 normal adult organs by this system revealed that 5' HOX genes were expressed in organs in the caudal parts of the body, and that the more caudal regions the more numbers of HOX genes were expressed. It was also found that the expression patterns of HOX genes were more similar in the adjacent genes on the same cluster rather than in those belonging to the same paralogs. Compared with normal thyroid tissues, thyroid cancer cell lines showed the altered expression of some HOX genes, especially Abd-B homeobox family genes. Our results showed that HOX genes were organ-specifically expressed in adult body and that the deregulated expressions of Abd-B family genes were implicated in thyroid tumor development.
Volume 293(1)
Pages 144-53
Published 2004-2-1
DOI 10.1016/j.yexcr.2003.09.024
PII S001448270300510X
PMID 14729064
MeSH Actins / genetics Actins / metabolism Adult Carcinoma / genetics* Carcinoma / pathology Cell Line, Tumor Cells, Cultured Computer Systems Gene Expression* Gene Expression Profiling* Genes, Homeobox* Glycerolphosphate Dehydrogenase / genetics Glycerolphosphate Dehydrogenase / metabolism Humans Kidney / cytology Kidney / embryology Lung Neoplasms / genetics Lung Neoplasms / pathology Organ Specificity RNA, Messenger / genetics RNA, Messenger / metabolism Reverse Transcriptase Polymerase Chain Reaction / methods Thyroid Gland / metabolism Thyroid Neoplasms / genetics* Thyroid Neoplasms / pathology
IF 3.383
Times Cited 111
Human and Animal Cells AKI(RCB0408)