論文 - 詳細
| RRC ID | 6495 |
|---|---|
| 著者 | Tsutsumida A, Hamada J, Tada M, Aoyama T, Furuuchi K, Kawai Y, Yamamoto Y, Sugihara T, Moriuchi T. |
| タイトル | Epigenetic silencing of E- and P-cadherin gene expression in human melanoma cell lines. |
| ジャーナル | Int J Oncol |
| Abstract |
The degree of E- and P-cadherin expressions inversely correlate with the progression stage of human melanoma. In the present study, we analyzed mechanisms of down-regulation of E- and P-cadherin gene expressions in 8 human melanoma cell lines. In 5 of the 8 melanoma cell lines, E-cadherin expression was lost or markedly decreased compared to that in normal melanocytes, and 4 of the 5 melanoma cell lines lost P-cadherin expression. All of the melanoma cell lines expressed snail, which is known to encode a transcription repressor for E-cadherin, at a higher level than melanocytes whereas expression levels of the snail varied among cell lines. Transduction of snail gene into MMAc cells which expressed a high level of E-cadherin and an extremely low level of snail decreased expression of E-cadherin but not P-cadherin. In contrast, transduction of antisense-snail gene into A375M cells which expressed no E-cadherin and a high level of snail restored expression of E-cadherin but not P-cadherin. Methylation-specific PCR analysis revealed CpG methylation in the promoter region of E-cadherin of MeWo and AKI cells. Further, the treatment with a demethylating agent, 5-azacytidine led AKI and A375M cells to re-express both E- and P-cadherin. The results show E-cadherin gene is silenced by at least two distinct mechanisms (methylation and transrepression by Snail) in human melanoma cell lines whereas P-cadherin gene seems to be silenced by methylation but not by snail. |
| 巻・号 | 25(5) |
| ページ | 1415-21 |
| 公開日 | 2004-11-1 |
| PMID | 15492833 |
| MeSH | Cadherins / biosynthesis* DNA Methylation DNA-Binding Proteins / pharmacology Down-Regulation Gene Expression Profiling* Gene Silencing* Humans Melanoma / genetics* Melanoma / pathology* Promoter Regions, Genetic Skin Neoplasms / genetics* Skin Neoplasms / pathology* Snail Family Transcription Factors Transcription Factors / pharmacology Tumor Cells, Cultured Zinc Fingers |
| IF | 3.899 |
| 引用数 | 25 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | AKI(RCB0408) MMAc(RCB0808) |