RRC ID 64969
Author Stevens MM, Maire CL, Chou N, Murakami MA, Knoff DS, Kikuchi Y, Kimmerling RJ, Liu H, Haidar S, Calistri NL, Cermak N, Olcum S, Cordero NA, Idbaih A, Wen PY, Weinstock DM, Ligon KL, Manalis SR.
Title Drug sensitivity of single cancer cells is predicted by changes in mass accumulation rate.
Journal Nat Biotechnol
Abstract Assays that can determine the response of tumor cells to cancer therapeutics could greatly aid the selection of drug regimens for individual patients. However, the utility of current functional assays is limited, and predictive genetic biomarkers are available for only a small fraction of cancer therapies. We found that the single-cell mass accumulation rate (MAR), profiled over many hours with a suspended microchannel resonator, accurately defined the drug sensitivity or resistance of glioblastoma and B-cell acute lymphocytic leukemia cells. MAR revealed heterogeneity in drug sensitivity not only between different tumors, but also within individual tumors and tumor-derived cell lines. MAR measurement predicted drug response using samples as small as 25 μl of peripheral blood while maintaining cell viability and compatibility with downstream characterization. MAR measurement is a promising approach for directly assaying single-cell therapeutic responses and for identifying cellular subpopulations with phenotypic resistance in heterogeneous tumors.
Volume 34(11)
Pages 1161-1167
Published 2016-11-1
DOI 10.1038/nbt.3697
PII nbt.3697
PMID 27723727
PMC PMC5142231
MeSH Antineoplastic Agents / administration & dosage* Cell Proliferation / drug effects Cells, Cultured Dose-Response Relationship, Drug Drug Resistance, Neoplasm Drug Screening Assays, Antitumor / instrumentation* Drug Screening Assays, Antitumor / methods Equipment Design Equipment Failure Analysis Humans Lab-On-A-Chip Devices* Micro-Electrical-Mechanical Systems / instrumentation* Micro-Electrical-Mechanical Systems / methods Neoplasms, Experimental / drug therapy* Neoplasms, Experimental / pathology Neoplasms, Experimental / physiopathology* Treatment Outcome
IF 36.553
Human and Animal Cells Ba/F3