RRC ID |
6498
|
著者 |
Uraguchi M, Morikawa M, Shirakawa M, Sanada K, Imai K.
|
タイトル |
Activation of WNT family expression and signaling in squamous cell carcinomas of the oral cavity.
|
ジャーナル |
J Dent Res
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Abstract |
The WNT family activates an oncogenic signaling mediated through beta-catenin and is up-regulated in a variety of malignant neoplasms. The signaling translocates beta-catenin into the nucleus and stimulates carcinoma cells in the epithelial-mesenchymal transition (EMT). However, WNT expression and signaling in oral carcinomas have not been examined. The present study focused on unveiling the involvement of WNTs in oral carcinomas, and showed that carcinoma cells express 11 of 19 WNT family members by reverse-transcription/PCR. WNT-expressing carcinoma cells exhibited increased beta-catenin levels in the cytoplasmic pool and translocation to the nucleus. The activation state of signaling correlated with the expression of membrane-type 1 matrix metalloproteinase, which degrades territorial matrices in carcinoma invasion. Immunohistochemistry disclosed that WNT3 expression and nuclear localization of beta-catenin were predominant in carcinoma cells at the invasive front. These results suggest that enhanced WNT expression and signaling accelerate the progression of carcinomas via activating EMTs and local invasiveness.
|
巻・号 |
83(4)
|
ページ |
327-32
|
公開日 |
2004-4-1
|
DOI |
10.1177/154405910408300411
|
PMID |
15044508
|
MeSH |
Carcinoma, Squamous Cell / genetics*
Carcinoma, Squamous Cell / metabolism
Cytoskeletal Proteins / metabolism
Gene Expression Profiling*
Gene Expression Regulation, Neoplastic*
Humans
Mouth Neoplasms / genetics*
Mouth Neoplasms / metabolism
Multigene Family
Proto-Oncogene Proteins / genetics*
Proto-Oncogene Proteins / metabolism
Signal Transduction
Trans-Activators / metabolism
Tumor Cells, Cultured
Wnt Proteins
Zebrafish Proteins*
beta Catenin
|
IF |
4.914
|
引用数 |
64
|
WOS 分野
|
DENTISTRY, ORAL SURGERY & MEDICINE
|
リソース情報 |
ヒト・動物細胞 |
|