RRC ID 6498
著者 Uraguchi M, Morikawa M, Shirakawa M, Sanada K, Imai K.
タイトル Activation of WNT family expression and signaling in squamous cell carcinomas of the oral cavity.
ジャーナル J Dent Res
Abstract The WNT family activates an oncogenic signaling mediated through beta-catenin and is up-regulated in a variety of malignant neoplasms. The signaling translocates beta-catenin into the nucleus and stimulates carcinoma cells in the epithelial-mesenchymal transition (EMT). However, WNT expression and signaling in oral carcinomas have not been examined. The present study focused on unveiling the involvement of WNTs in oral carcinomas, and showed that carcinoma cells express 11 of 19 WNT family members by reverse-transcription/PCR. WNT-expressing carcinoma cells exhibited increased beta-catenin levels in the cytoplasmic pool and translocation to the nucleus. The activation state of signaling correlated with the expression of membrane-type 1 matrix metalloproteinase, which degrades territorial matrices in carcinoma invasion. Immunohistochemistry disclosed that WNT3 expression and nuclear localization of beta-catenin were predominant in carcinoma cells at the invasive front. These results suggest that enhanced WNT expression and signaling accelerate the progression of carcinomas via activating EMTs and local invasiveness.
巻・号 83(4)
ページ 327-32
公開日 2004-4-1
DOI 10.1177/154405910408300411
PMID 15044508
MeSH Carcinoma, Squamous Cell / genetics* Carcinoma, Squamous Cell / metabolism Cytoskeletal Proteins / metabolism Gene Expression Profiling* Gene Expression Regulation, Neoplastic* Humans Mouth Neoplasms / genetics* Mouth Neoplasms / metabolism Multigene Family Proto-Oncogene Proteins / genetics* Proto-Oncogene Proteins / metabolism Signal Transduction Trans-Activators / metabolism Tumor Cells, Cultured Wnt Proteins Zebrafish Proteins* beta Catenin
IF 4.914
引用数 64
WOS 分野 DENTISTRY, ORAL SURGERY & MEDICINE
リソース情報
ヒト・動物細胞