RRC ID 65024
Author Sasamoto T, Fujimoto K, Kanawa M, Kimura J, Takeuchi J, Harada N, Goto N, Kawamoto T, Noshiro M, Suardita K, Tanne K, Kato Y.
Title DEC2 is a negative regulator for the proliferation and differentiation of chondrocyte lineage-committed mesenchymal stem cells.
Journal Int J Mol Med
Abstract Differentiated embryo chondrocyte 2 (DEC2) is a basic helix-loop-helix-Orange transcription factor that regulates cell differentiation in various mammalian tissues. DEC2 has been shown to suppress the differentiation of mesenchymal stem cells (MSCs) into myocytes and adipocytes. In the present study, we examined the role of DEC2 in the chondrogenic differentiation of human MSCs. The overexpression of DEC2 exerted minimal effects on the proliferation of MSCs in monolayer cultures with the growth medium under undifferentiating conditions, whereas it suppressed increases in DNA content, glycosaminoglycan content, and the expression of several chondrocyte-related genes, including aggrecan and type X collagen alpha 1, in MSC pellets in centrifuge tubes under chondrogenic conditions. In the pellets exposed to chondrogenesis induction medium, DEC2 overexpression downregulated the mRNA expression of fibroblast growth factor 18, which is involved in the proliferation and differentiation of chondrocytes, and upregulated the expression of p16INK4, which is a cell cycle inhibitor. These findings suggest that DEC2 is a negative regulator of the proliferation and differentiation of chondrocyte lineage-committed mesenchymal cells.
Volume 38(3)
Pages 876-84
Published 2016-9-1
DOI 10.3892/ijmm.2016.2660
PMID 27430159
MeSH Aggrecans / genetics Animals Basic Helix-Loop-Helix Transcription Factors / genetics* Basic Helix-Loop-Helix Transcription Factors / metabolism Blotting, Western Cell Cycle / genetics Cell Differentiation / genetics* Cell Lineage / genetics Cell Proliferation / genetics* Cells, Cultured Chondrocytes / cytology Chondrocytes / metabolism* Collagen Type X / genetics Cyclin-Dependent Kinase Inhibitor p16 / genetics DNA / genetics DNA / metabolism Extracellular Matrix / metabolism Fibroblast Growth Factors / genetics Gene Expression Regulation Glycosaminoglycans / metabolism Humans Mesenchymal Stem Cells / cytology Mesenchymal Stem Cells / metabolism* Mice Reverse Transcriptase Polymerase Chain Reaction
IF 3.098
Human and Animal Cells Human mesenchymal stem cells