RRC ID 65042
Author Okamura E, Ohtaka K, Nishihama R, Uchida K, Kuwahara A, Mochida K, Hirai MY.
Title Diversified amino acid-mediated allosteric regulation of phosphoglycerate dehydrogenase for serine biosynthesis in land plants.
Journal Biochem J
Abstract The phosphorylated pathway of serine biosynthesis is initiated with 3-phosphoglycerate dehydrogenase (PGDH). The liverwort Marchantia polymorpha possesses an amino acid-sensitive MpPGDH which is inhibited by L-serine and activated by five proteinogenic amino acids, while the eudicot Arabidopsis thaliana has amino acid-sensitive AtPGDH1 and AtPGDH3 as well as amino acid-insensitive AtPGDH2. In this study, we analyzed PGDH isozymes of the representative land plants: the monocot Oryza sativa (OsPGDH1‒3), basal angiosperm Amborella trichopoda (AmtriPGDH1‒2), and moss Physcomitrium (Physcomitrella) patens (PpPGDH1‒4). We demonstrated that OsPGDH1, AmtriPGDH1, PpPGDH1, and PpPGDH3 were amino acid-sensitive, whereas OsPGDH2, OsPGDH3, AmtriPGDH2, PpPGDH2 and PpPGDH4 were either sensitive to only some of the six effector amino acids or insensitive to all effectors. This indicates that PGDH sensitivity to effectors has been diversified among isozymes and that the land plant species examined, except for M. polymorpha, possess different isozyme types in terms of regulation. Phylogenetic analysis suggested that the different sensitivities convergently evolved in the bryophyte and angiosperm lineages. Site-directed mutagenesis of AtPGDH1 revealed that Asp538 and Asn556 residues in the ACT domain are involved in allosteric regulation by the effectors. These findings provide insight into the evolution of PGDH isozymes, highlighting the functional diversification of allosteric regulation in land plants.
Volume 478(12)
Pages 2217-2232
Published 2021-6-25
DOI 10.1042/BCJ20210191
PII 228733
PMID 34032263
PMC PMC8238522
MeSH Allosteric Regulation Amino Acid Sequence Arabidopsis / enzymology Bryopsida / enzymology Gene Expression Regulation, Plant* Marchantia / enzymology Mutation* Oryza / enzymology Phosphoglycerate Dehydrogenase / chemistry Phosphoglycerate Dehydrogenase / genetics Phosphoglycerate Dehydrogenase / metabolism* Phylogeny Plant Proteins / chemistry Plant Proteins / genetics Plant Proteins / metabolism* Sequence Homology Serine / biosynthesis*
IF 4.097
Arabidopsis / Cultured plant cells, genes pdp33831 pdp82465 pdp12194