論文 - 詳細
| RRC ID | 65067 |
|---|---|
| 著者 | Hama K, Fujiwara Y, Tabata H, Takahashi H, Yokoyama K. |
| タイトル | Comprehensive Quantitation Using Two Stable Isotopically Labeled Species and Direct Detection of N-Acyl Moiety of Sphingomyelin. |
| ジャーナル | Lipids |
| Abstract |
Sphingomyelin (ceramide-phosphocholine, CerPCho) is a common sphingolipid in mammalian cells and is composed of phosphorylcholine and ceramide as polar and hydrophobic components, respectively. In this study, a qualitative liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS/MS) analysis is proposed in which CerPCho structures were assigned based on product ion spectra corresponding to sphingosylphosphorylcholine and N-acyl moieties. From MS/MS/MS analysis of CerPCho, we observed product ion spectra of the N-acyl fatty acids as [RCO2]- ions as well as sphingosylphosphorylcholine. A calibration curve for CerPCho was constructed using two stable isotopically labeled CerPCho species and then used to quantify the CerPCho species in HeLa cells as a proof-of-principle study. The present study proposes an accurate method for quantifying and assigning structures to each CerPCho species in crude biologic samples by LC-ESI-MS/MS/MS analysis. |
| 巻・号 | 52(9) |
| ページ | 789-799 |
| 公開日 | 2017-9-1 |
| DOI | 10.1007/s11745-017-4279-5 |
| PII | 10.1007/s11745-017-4279-5 |
| PMID | 28770378 |
| PMC | PMC5583271 |
| MeSH | Acetyltransferases / genetics Acetyltransferases / metabolism Acylation Chromatography, Liquid Fatty Acid Elongases HeLa Cells Humans Spectrometry, Mass, Electrospray Ionization Sphingomyelins / analysis* Sphingomyelins / metabolism Tandem Mass Spectrometry Up-Regulation |
| IF | 1.919 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |