| RRC ID |
6506
|
| Author |
Tsuji AB, Sugyo A, Sudo H, Sagara M, Ishikawa A, Ohtsuki M, Kimura T, Ogiu T, Miyagishi M, Taira K, Imai T, Harada YN.
|
| Title |
Defective repair of radiation-induced DNA damage is complemented by a CHORI-230-65K18 BAC clone on rat chromosome 4.
|
| Journal |
Genomics
|
| Abstract |
The Long Evans cinnamon (LEC) rat is highly susceptible to X-irradiation due to defective DNA repair and is thus a model for hepatocellular carcinogenesis. We constructed a bacterial artificial chromosome (BAC) contig of rat chromosome 4 completely covering the region associated with radiation susceptibility. We used transient and stable transfections to demonstrate that defective DNA repair in LEC cells is fully complemented by a 200-kb BAC, CHORI-230-65K18. Further analysis showed that the region associated with radiation susceptibility is located in a 128,543-bp region of 65K18 that includes the known gene Rpn1. However, neither knockdown nor overexpression of Rpn1 indicated that this gene is associated with radiation susceptibility. We also mapped three ESTs (TC523872, TC533727, and CB607546) in the 128,543-bp region, suggesting that 65K18 contains an unknown gene associated with X-ray susceptibility in the LEC rat.
|
| Volume |
87(2)
|
| Pages |
236-42
|
| Published |
2006-2-1
|
| DOI |
10.1016/j.ygeno.2005.09.020
|
| PII |
S0888-7543(05)00288-0
|
| PMID |
16309880
|
| MeSH |
Animals
Base Sequence
Cell Line
Chromosome Mapping*
Clone Cells
DNA Damage*
DNA Primers
DNA Repair*
Genetic Complementation Test
Radiation Tolerance / genetics
Rats
Transfection
X-Rays
|
| IF |
6.205
|
| Times Cited |
0
|
|
WOS Category
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
GENETICS & HEREDITY
|
| Resource |
| Human and Animal Cells |
3Y1-B clone 1-6(RCB0488) |
