RRC ID 65079
Author Ogawa Y, Noda K, Kimura S, Kitaoka M, Wada M.
Title Facile preparation of highly crystalline lamellae of (1 → 3)-β-D-glucan using an extract of Euglena gracilis.
Journal Int J Biol Macromol
Abstract In vitro synthesis of (1 → 3)-β-D-glucan was performed using laminaribiose phosphorylase obtained by an extraction of Euglena gracilis with sucrose phosphorylase. The synthetic product was a linear (1 → 3)-β-D-glucan with a narrow distribution of degree of polymerization (DP) centered on DP=30. X-ray diffraction and electron microscopy revealed that the glucan molecules obtained were self-organized as highly crystalline hexagonal lamellae. This synthetic product has quite high structural homogeneity at every level from primary to higher-order structure, which is a great advantage for the detailed analyses of physiological functions of (1 → 3)-β-D-glucan.
Volume 64
Pages 415-9
Published 2014-3-1
DOI 10.1016/j.ijbiomac.2013.12.027
PII S0141-8130(13)00685-5
PMID 24374085
MeSH Euglena gracilis / chemistry* Glucosyltransferases / chemistry* Glucosyltransferases / isolation & purification Microscopy, Electron X-Ray Diffraction beta-Glucans / chemical synthesis*
IF 4.784
Resource
Algae NIES-48