RRC ID |
65079
|
著者 |
Ogawa Y, Noda K, Kimura S, Kitaoka M, Wada M.
|
タイトル |
Facile preparation of highly crystalline lamellae of (1 → 3)-β-D-glucan using an extract of Euglena gracilis.
|
ジャーナル |
Int J Biol Macromol
|
Abstract |
In vitro synthesis of (1 → 3)-β-D-glucan was performed using laminaribiose phosphorylase obtained by an extraction of Euglena gracilis with sucrose phosphorylase. The synthetic product was a linear (1 → 3)-β-D-glucan with a narrow distribution of degree of polymerization (DP) centered on DP=30. X-ray diffraction and electron microscopy revealed that the glucan molecules obtained were self-organized as highly crystalline hexagonal lamellae. This synthetic product has quite high structural homogeneity at every level from primary to higher-order structure, which is a great advantage for the detailed analyses of physiological functions of (1 → 3)-β-D-glucan.
|
巻・号 |
64
|
ページ |
415-9
|
公開日 |
2014-3-1
|
DOI |
10.1016/j.ijbiomac.2013.12.027
|
PII |
S0141-8130(13)00685-5
|
PMID |
24374085
|
MeSH |
Euglena gracilis / chemistry*
Glucosyltransferases / chemistry*
Glucosyltransferases / isolation & purification
Microscopy, Electron
Proteoglycans
X-Ray Diffraction
beta-Glucans / chemical synthesis*
|
IF |
5.162
|
リソース情報 |
藻類 |
NIES-48 |