論文 - 詳細
| RRC ID | 65109 |
|---|---|
| 著者 | Guo M, Xu W, Yamamoto Y, Suzuki T. |
| タイトル | Curcumin increases heat shock protein 70 expression via different signaling pathways in intestinal epithelial cells. |
| ジャーナル | Arch Biochem Biophys |
| Abstract |
Intestinal inflammation is associated with the integrity of the intestinal epithelium, which forms a physical barrier against noxious luminal substances. Heat shock 70 kDa protein 1A (HSP70), a molecular chaperon that exerts a cytoprotective effect, regulates intestinal integrity. This study investigated the modulation of HSP70 expression by dietary polyphenols, with particular reference to curcumin, in human intestinal Caco-2 cells. Immunoblot analysis demonstrated that among the 21 different polyphenols tested, curcumin most potently increased HSP70 levels in Caco-2 cells without affecting cell viability. Curcumin also increased the phosphorylation of heat shock factor 1 (HSF1), a well-known transcription factor of HSP70. Promoter and qRT-PCR assays indicated that curcumin upregulated Hspa1a levels via transcriptional activation. Pharmacological inhibition of MEK, a mechanistic target of rapamycin, p38 mitogen-activated protein kinase, and phosphatidyl 3-inositol kinase suppressed curcumin-mediated HSP70 expression, whereas HSF1 phosphorylation was sensitive only to MEK inhibition. Taken together, curcumin increases the expression of HSP70 in intestinal Caco-2 cells via transcriptional activation, possibly enhancing cell integrity. The effects exerted by curcumin are regulated by various signaling pathways. Our findings will expectedly contribute to a deeper understanding of the regulation of intestinal HSP70 by dietary components. |
| 巻・号 | 707 |
| ページ | 108938 |
| 公開日 | 2021-8-15 |
| DOI | 10.1016/j.abb.2021.108938 |
| PII | S0003-9861(21)00187-9 |
| PMID | 34051214 |
| MeSH | Caco-2 Cells Cell Survival / drug effects Curcumin / pharmacology* Gene Expression Regulation / drug effects* HSP70 Heat-Shock Proteins / metabolism* Humans Intestinal Mucosa / cytology* Intestinal Mucosa / drug effects* Phosphorylation / drug effects Signal Transduction / drug effects* |
| IF | 3.391 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | IEC 6(RCB0993) |