論文 - 詳細
| RRC ID | 65114 |
|---|---|
| 著者 | Sahoo PR, Kowada T, Mizukami S. |
| タイトル | Long-Term Mg2+ Imaging in Live Cells with a Targetable Fluorescent Probe. |
| ジャーナル | Methods Mol Biol |
| Abstract |
Living cells dynamically change their morphology and function according to the cell cycle. Long-term observations of living cells are privileged when we spy the unique, cell cycle-driven molecular events, which cannot be obtained from short-term ones. Mg2+, a metal ion abundant in cells, has been shown to be involved in a variety of physiological phenomena by noninvasive cellular observation using fluorescence microscopy. However, long-term observation of Mg2+ in cells has been a great challenge. Herein, we present a protocol for the long-term microscopic imaging of intracellular Mg2+ levels using a small molecule-protein hybrid fluorescent probe we developed. |
| 巻・号 | 2274 |
| ページ | 237-243 |
| 公開日 | 2021-1-1 |
| DOI | 10.1007/978-1-0716-1258-3_20 |
| PMID | 34050476 |
| MeSH | Fluorescent Dyes / chemistry* HEK293 Cells Humans Magnesium / metabolism* Microscopy, Fluorescence / methods Molecular Imaging / methods* Signal Transduction |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 293T(RCB2202) |