RRC ID 65114
Author Sahoo PR, Kowada T, Mizukami S.
Title Long-Term Mg2+ Imaging in Live Cells with a Targetable Fluorescent Probe.
Journal Methods Mol Biol
Abstract Living cells dynamically change their morphology and function according to the cell cycle. Long-term observations of living cells are privileged when we spy the unique, cell cycle-driven molecular events, which cannot be obtained from short-term ones. Mg2+, a metal ion abundant in cells, has been shown to be involved in a variety of physiological phenomena by noninvasive cellular observation using fluorescence microscopy. However, long-term observation of Mg2+ in cells has been a great challenge. Herein, we present a protocol for the long-term microscopic imaging of intracellular Mg2+ levels using a small molecule-protein hybrid fluorescent probe we developed.
Volume 2274
Pages 237-243
Published 2021-1-1
DOI 10.1007/978-1-0716-1258-3_20
PMID 34050476
MeSH Fluorescent Dyes / chemistry* HEK293 Cells Humans Magnesium / metabolism* Microscopy, Fluorescence / methods Molecular Imaging / methods* Signal Transduction
Human and Animal Cells 293T(RCB2202)