| RRC ID |
65164
|
| 著者 |
Hirai T, Kono K, Sawada R, Kuroda T, Yasuda S, Matsuyama S, Matsuyama A, Koizumi N, Utoguchi N, Mizuguchi H, Sato Y.
|
| タイトル |
A selective cytotoxic adenovirus vector for concentration of pluripotent stem cells in human pluripotent stem cell-derived neural progenitor cells.
|
| ジャーナル |
Sci Rep
|
| Abstract |
Highly sensitive detection of residual undifferentiated pluripotent stem cells is essential for the quality and safety of cell-processed therapeutic products derived from human induced pluripotent stem cells (hiPSCs). We previously reported the generation of an adenovirus (Ad) vector and adeno-associated virus vectors that possess a suicide gene, inducible Caspase 9 (iCasp9), which makes it possible to sensitively detect undifferentiated hiPSCs in cultures of hiPSC-derived cardiomyocytes. In this study, we investigated whether these vectors also allow for detection of undifferentiated hiPSCs in preparations of hiPSC-derived neural progenitor cells (hiPSC-NPCs), which have been expected to treat neurological disorders. To detect undifferentiated hiPSCs, the expression of pluripotent stem cell markers was determined by immunostaining and flow cytometry. Using immortalized NPCs as a model, the Ad vector was identified to be the most efficient among the vectors tested in detecting undifferentiated hiPSCs. Moreover, we found that the Ad vector killed most hiPSC-NPCs in an iCasp9-dependent manner, enabling flow cytometry to detect undifferentiated hiPSCs intermingled at a lower concentration (0.002%) than reported previously (0.1%). These data indicate that the Ad vector selectively eliminates hiPSC-NPCs, thus allowing for sensitive detection of hiPSCs. This cytotoxic viral vector could contribute to ensuring the quality and safety of hiPSCs-NPCs for therapeutic use.
|
| 巻・号 |
11(1)
|
| ページ |
11407
|
| 公開日 |
2021-6-1
|
| DOI |
10.1038/s41598-021-90928-7
|
| PII |
10.1038/s41598-021-90928-7
|
| PMID |
34075124
|
| PMC |
PMC8169681
|
| MeSH |
Adenoviridae / genetics
Cell Differentiation
Cells, Cultured
Genetic Vectors
Humans
Induced Pluripotent Stem Cells / cytology*
Myocytes, Cardiac / cytology*
Neural Stem Cells / cytology*
|
| IF |
3.998
|
| リソース情報 |
| ヒト・動物細胞 |
253G1(HPS0002) |
