論文 - 詳細
| RRC ID | 65164 |
|---|---|
| 著者 | Hirai T, Kono K, Sawada R, Kuroda T, Yasuda S, Matsuyama S, Matsuyama A, Koizumi N, Utoguchi N, Mizuguchi H, Sato Y. |
| タイトル | A selective cytotoxic adenovirus vector for concentration of pluripotent stem cells in human pluripotent stem cell-derived neural progenitor cells. |
| ジャーナル | Sci Rep |
| Abstract |
Highly sensitive detection of residual undifferentiated pluripotent stem cells is essential for the quality and safety of cell-processed therapeutic products derived from human induced pluripotent stem cells (hiPSCs). We previously reported the generation of an adenovirus (Ad) vector and adeno-associated virus vectors that possess a suicide gene, inducible Caspase 9 (iCasp9), which makes it possible to sensitively detect undifferentiated hiPSCs in cultures of hiPSC-derived cardiomyocytes. In this study, we investigated whether these vectors also allow for detection of undifferentiated hiPSCs in preparations of hiPSC-derived neural progenitor cells (hiPSC-NPCs), which have been expected to treat neurological disorders. To detect undifferentiated hiPSCs, the expression of pluripotent stem cell markers was determined by immunostaining and flow cytometry. Using immortalized NPCs as a model, the Ad vector was identified to be the most efficient among the vectors tested in detecting undifferentiated hiPSCs. Moreover, we found that the Ad vector killed most hiPSC-NPCs in an iCasp9-dependent manner, enabling flow cytometry to detect undifferentiated hiPSCs intermingled at a lower concentration (0.002%) than reported previously (0.1%). These data indicate that the Ad vector selectively eliminates hiPSC-NPCs, thus allowing for sensitive detection of hiPSCs. This cytotoxic viral vector could contribute to ensuring the quality and safety of hiPSCs-NPCs for therapeutic use. |
| 巻・号 | 11(1) |
| ページ | 11407 |
| 公開日 | 2021-6-1 |
| DOI | 10.1038/s41598-021-90928-7 |
| PII | 10.1038/s41598-021-90928-7 |
| PMID | 34075124 |
| PMC | PMC8169681 |
| MeSH | Adenoviridae / genetics Cell Differentiation Cells, Cultured Genetic Vectors Humans Induced Pluripotent Stem Cells / cytology* Myocytes, Cardiac / cytology* Neural Stem Cells / cytology* |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 253G1(HPS0002) |