RRC ID 65173
Author Zhang M, Li Z, Gao D, Gong W, Gao Y, Zhang C.
Title Hydrogen extends Caenorhabditis elegans longevity by reducing reactive oxygen species.
Journal PLoS One
Abstract At present, a large number of studies have reported that hydrogen has antioxidant functions and prevents oxidative stress damage. However, it is not clear whether hydrogen can prolong longevity based on these effects. Therefore, we studied and explored the antiaging potential of exogenous hydrogen and its ability to extend longevity using Caenorhabditis elegans (C. elegans) as an animal model. Our results showed that the lifespans of the N2, sod-3 and sod-5 mutant strains were extended by approximately 22.7%, 9.5%, and 8.7%, respectively, after hydrogen treatment, but hydrogen had no effect on the lifespans of the daf-2 and daf-16 mutant strains. Meanwhile, the level of reactive oxygen species (ROS) in the hydrogen treatment group was significantly lower than that in the control group. At the transcript level, the expression of age-1 and let-363 was obviously decreased, while the expression of ins-18 was increased at the same time point (14 d). Compared with the control group, paraquat (PQ) could reduce the lifespan of the N2 and sod-5 mutant strains. Importantly, the longevity of these mutant strains recovered to normal levels when the animals were treated with exogenous hydrogen. According to these results, the lifespan of C. elegans is closely related to oxidative stress and can be significantly prolonged by reducing oxidative stress damage. Taken together, our data showed that hydrogen is a valuable antioxidant that can significantly reduce the body's ROS levels and extend the lifespan of C. elegans. This study also laid a foundation for the subsequent application of hydrogen in antiaging studies.
Volume 15(4)
Pages e0231972
Published 2020-1-1
DOI 10.1371/journal.pone.0231972
PII PONE-D-19-32129
PMID 32320994
PMC PMC7176462
MeSH Animals Caenorhabditis elegans / cytology Caenorhabditis elegans / drug effects* Caenorhabditis elegans / metabolism Caenorhabditis elegans / physiology* Down-Regulation / drug effects Drug Interactions Hydrogen / pharmacology* Insulin / metabolism Insulin-Like Growth Factor I / metabolism Longevity / drug effects* Oxidative Stress / drug effects Paraquat / pharmacology Reactive Oxygen Species / metabolism* Signal Transduction / drug effects
Resource
C.elegans tm1146