RRC ID 6518
著者 Kubo Y, Akiyama M, Fukada Y, Okano T.
タイトル Molecular cloning, mRNA expression, and immunocytochemical localization of a putative blue-light photoreceptor CRY4 in the chicken pineal gland.
ジャーナル J Neurochem
Abstract In non-mammalian vertebrates, the pineal gland contains an endogenous circadian oscillator and serves as a photosensitive neuroendocrinal organ. To better understand the pineal phototransduction mechanism, we focused on the chicken putative blue-light photoreceptive molecule, Cryptochrome4 (cCRY4). Here we report the molecular cloning of pineal cCry4 cDNA, the in vivo expression of cCry4 mRNA, and the detection of cCRY4 protein. cCry4 is transcribed in a wide variety of chick tissues out of which the pineal gland and retina contain high levels of cCry4 mRNA. In the pineal gland, under 12 h light : 12 h dark cycles, the levels of both cCry4 mRNA and cCRY4 protein showed diurnal changes, and in cultured chick pineal cells, the cCry4 mRNA level was not only up-regulated by light but also controlled by circadian signals. Immunoblot analysis with a cCRY4-specific antibody detected cCRY4 in a soluble fraction of the pineal lysate. Immunocytochemistry revealed that cCRY4 was expressed in many parenchymal cells and a limited number of stromal cells. These cCRY4 features strikingly contrast with those of the chick pineal photoreceptor pinopsin, suggesting a possible temporal and/or spatial duplicity of the pineal photoreceptive system, the opsin- and CRY-based mechanisms.
巻・号 97(4)
ページ 1155-65
公開日 2006-5-1
DOI 10.1111/j.1471-4159.2006.03826.x
PII JNC3826
PMID 16686694
MeSH Animals Cell Line Chickens Circadian Rhythm / physiology* Circadian Rhythm / radiation effects Cloning, Molecular Cryptochromes DNA, Complementary / analysis DNA, Complementary / genetics Flavoproteins / genetics Flavoproteins / isolation & purification Flavoproteins / metabolism* Humans Light Light Signal Transduction / physiology* Light Signal Transduction / radiation effects Male Molecular Sequence Data Pineal Gland / cytology Pineal Gland / metabolism* Pineal Gland / radiation effects RNA, Messenger / genetics RNA, Messenger / metabolism RNA, Messenger / radiation effects Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Up-Regulation / genetics Up-Regulation / radiation effects
IF 4.066
引用数 17
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY NEUROSCIENCES
リソース情報
ヒト・動物細胞 293(RCB1637)