Reference - Detail
| RRC ID | 65189 |
|---|---|
| Author | Jeong PY, Kumar A, Joshi PM, Rothman JH. |
| Title | Intertwined Functions of Separase and Caspase in Cell Division and Programmed Cell Death. |
| Journal | Sci Rep |
| Abstract |
Timely sister chromatid separation, promoted by separase, is essential for faithful chromosome segregation. Separase is a member of the CD clan of cysteine proteases, which also includes the pro-apoptotic enzymes known as caspases. We report a role for the C. elegans separase SEP-1, primarily known for its essential activity in cell division and cortical granule exocytosis, in developmentally programmed cell death when the predominant pro-apoptotic caspase CED-3 is compromised. Loss of SEP-1 results in extra surviving cells in a weak ced-3(-) mutant, and suppresses the embryonic lethality of a mutant defective for the apoptotic suppressor ced-9/Bcl-2 implicating SEP-1 in execution of apoptosis. We also report apparent non-apoptotic roles for CED-3 in promoting germ cell proliferation, meiotic chromosome disjunction, egg shell formation, and the normal rate of embryonic development. Moreover, loss of the soma-specific (CSP-3) and germline-specific (CSP-2) caspase inhibitors result in CED-3-dependent suppression of embryonic lethality and meiotic chromosome non-disjunction respectively, when separase function is compromised. Thus, while caspases and separases have evolved different substrate specificities associated with their specialized functions in apoptosis and cell division respectively, they appear to have retained the residual ability to participate in both processes, supporting the view that co-option of components in cell division may have led to the innovation of programmed cell suicide early in metazoan evolution. |
| Volume | 10(1) |
| Pages | 6159 |
| Published | 2020-4-9 |
| DOI | 10.1038/s41598-020-63081-w |
| PII | 10.1038/s41598-020-63081-w |
| PMID | 32273538 |
| PMC | PMC7145830 |
| MeSH | Animals Apoptosis* / physiology Caenorhabditis elegans / enzymology Caenorhabditis elegans / growth & development Caenorhabditis elegans / metabolism Caenorhabditis elegans / physiology Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology Caspases / metabolism* Caspases / physiology Cell Division* / physiology Female Male Separase / metabolism* Separase / physiology |
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm2858 tm3077 tm2260 tm2486 |