論文 - 詳細
| RRC ID | 65339 |
|---|---|
| 著者 | Chen YC, Huang HR, Hsu CH, Ou CY. |
| タイトル | CRMP/UNC-33 organizes microtubule bundles for KIF5-mediated mitochondrial distribution to axon. |
| ジャーナル | PLoS Genet |
| Abstract |
Neurons are highly specialized cells with polarized cellular processes and subcellular domains. As vital organelles for neuronal functions, mitochondria are distributed by microtubule-based transport systems. Although the essential components of mitochondrial transport including motors and cargo adaptors are identified, it is less clear how mitochondrial distribution among somato-dendritic and axonal compartment is regulated. Here, we systematically study mitochondrial motors, including four kinesins, KIF5, KIF17, KIF1, KLP-6, and dynein, and transport regulators in C. elegans PVD neurons. Among all these motors, we found that mitochondrial export from soma to neurites is mainly mediated by KIF5/UNC-116. Interestingly, UNC-116 is especially important for axonal mitochondria, while dynein removes mitochondria from all plus-end dendrites and the axon. We surprisingly found one mitochondrial transport regulator for minus-end dendritic compartment, TRAK-1, and two mitochondrial transport regulators for axonal compartment, CRMP/UNC-33 and JIP3/UNC-16. While JIP3/UNC-16 suppresses axonal mitochondria, CRMP/UNC-33 is critical for axonal mitochondria; nearly no axonal mitochondria present in unc-33 mutants. We showed that UNC-33 is essential for organizing the population of UNC-116-associated microtubule bundles, which are tracks for mitochondrial trafficking. Disarrangement of these tracks impedes mitochondrial transport to the axon. In summary, we identified a compartment-specific transport regulation of mitochondria by UNC-33 through organizing microtubule tracks for different kinesin motors other than microtubule polarity. |
| 巻・号 | 17(2) |
| ページ | e1009360 |
| 公開日 | 2021-2-1 |
| DOI | 10.1371/journal.pgen.1009360 |
| PII | PGENETICS-D-20-01044 |
| PMID | 33571181 |
| PMC | PMC7904166 |
| MeSH | Animals Animals, Genetically Modified Axons / metabolism* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Dendrites / metabolism Dyneins / metabolism Kinesins / genetics Kinesins / metabolism* Microscopy, Confocal Microscopy, Fluorescence Microtubules / metabolism* Mitochondria / metabolism* Mutation Nerve Growth Factors / genetics Nerve Growth Factors / metabolism* Neurons / metabolism Protein Transport |
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オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1966 tm1572 |