| RRC ID |
65345
|
| Author |
Carpenter BS, Lee TW, Plott CF, Rodriguez JD, Brockett JS, Myrick DA, Katz DJ.
|
| Title |
Caenorhabditis elegans establishes germline versus soma by balancing inherited histone methylation.
|
| Journal |
Development
|
| Abstract |
Formation of a zygote is coupled with extensive epigenetic reprogramming to enable appropriate inheritance of histone methylation and prevent developmental delays. In Caenorhabditis elegans, this reprogramming is mediated by the H3K4me2 demethylase SPR-5 and the H3K9 methyltransferase, MET-2. In contrast, the H3K36 methyltransferase MES-4 maintains H3K36me2/3 at germline genes between generations to facilitate re-establishment of the germline. To determine whether the MES-4 germline inheritance pathway antagonizes spr-5; met-2 reprogramming, we examined the interaction between these two pathways. We found that the developmental delay of spr-5; met-2 mutant progeny is associated with ectopic H3K36me3 and the ectopic expression of MES-4-targeted germline genes in somatic tissues. Furthermore, the developmental delay is dependent upon MES-4 and the H3K4 methyltransferase, SET-2. We propose that MES-4 prevents crucial germline genes from being repressed by antagonizing maternal spr-5; met-2 reprogramming. Thus, the balance of inherited histone modifications is necessary to distinguish germline versus soma and prevent developmental delay.This article has an associated 'The people behind the papers' interview.
|
| Volume |
148(3)
|
| Published |
2021-2-10
|
| DOI |
10.1242/dev.196600
|
| PII |
dev.196600
|
| PMID |
33462111
|
| PMC |
PMC7888751
|
| MeSH |
Animals
Caenorhabditis elegans / genetics
Caenorhabditis elegans / metabolism*
Caenorhabditis elegans Proteins / metabolism
Carisoprodol / metabolism*
Epigenesis, Genetic
Epigenomics
Gene Expression
Gene Knockdown Techniques
Germ Cells / metabolism*
Histones / metabolism*
Methylation
Protein Processing, Post-Translational
|
| Resource |
| C.elegans |
|
