RRC ID 65388
Author Dinić M, Herholz M, Kačarević U, Radojević D, Novović K, Đokić J, Trifunović A, Golić N.
Title Host-commensal interaction promotes health and lifespan in Caenorhabditis elegans through the activation of HLH-30/TFEB-mediated autophagy.
Journal Aging (Albany NY)
Abstract Gut homeostasis is maintained by the close interaction between commensal intestinal microbiota and the host, affecting the most complex physiological processes, such as aging. Some commensal bacteria with the potential to promote healthy aging arise as attractive candidates for the development of pro-longevity probiotics. Here, we showed that heat-inactivated human commensal Lactobacillus fermentum BGHV110 (BGHV110) extends the lifespan of Caenorhabditis elegans and improves age-related physiological features, including locomotor function and lipid metabolism. Mechanistically, we found that BGHV110 promotes HLH-30/TFEB-dependent autophagy to delay aging, as longevity assurance was completely abolished in the mutant lacking HLH-30, a major autophagy regulator in C. elegans. Moreover, we observed that BGHV110 partially decreased the content of lipid droplets in an HLH-30-dependent manner and, at the same time, slightly increased mitochondrial activity. In summary, this study demonstrates that specific factors from commensal bacteria can be used to exploit HLH-30/TFEB-mediated autophagy in order to promote longevity and fitness of the host.
Volume 13(6)
Pages 8040-8054
Published 2021-3-26
DOI 10.18632/aging.202885
PII 202885
PMID 33770762
PMC PMC8034897
MeSH Aging / metabolism* Animals Autophagy / physiology* Basic Helix-Loop-Helix Transcription Factors / metabolism* Caenorhabditis elegans / metabolism* Caenorhabditis elegans / microbiology Caenorhabditis elegans Proteins / metabolism* Homeostasis / physiology Limosilactobacillus fermentum Lipid Metabolism / physiology Longevity / physiology* Mitochondria / metabolism
C.elegans tm1978