Reference - Detail
| RRC ID | 65398 |
|---|---|
| Author | Abrams J, Nance J. |
| Title | A polarity pathway for exocyst-dependent intracellular tube extension. |
| Journal | Elife |
| Abstract |
Lumen extension in intracellular tubes can occur when vesicles fuse with an invading apical membrane. Within the Caenorhabditis elegans excretory cell, which forms an intracellular tube, the exocyst vesicle-tethering complex is enriched at the lumenal membrane and is required for its outgrowth, suggesting that exocyst-targeted vesicles extend the lumen. Here, we identify a pathway that promotes intracellular tube extension by enriching the exocyst at the lumenal membrane. We show that PAR-6 and PKC-3/aPKC concentrate at the lumenal membrane and promote lumen extension. Using acute protein depletion, we find that PAR-6 is required for exocyst membrane recruitment, whereas PAR-3, which can recruit the exocyst in mammals, appears dispensable for exocyst localization and lumen extension. Finally, we show that CDC-42 and RhoGEF EXC-5/FGD regulate lumen extension by recruiting PAR-6 and PKC-3 to the lumenal membrane. Our findings reveal a pathway that connects CDC-42, PAR proteins, and the exocyst to extend intracellular tubes. |
| Volume | 10 |
| Published | 2021-3-9 |
| DOI | 10.7554/eLife.65169 |
| PII | 65169 |
| PMID | 33687331 |
| PMC | PMC8021397 |
| MeSH | Animals Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Cell Polarity* Cytoplasm / metabolism* Protein Kinase C / genetics* Protein Kinase C / metabolism |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 7 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm1443 tm5205 |