RRC ID 65416
著者 Gaspar N, Walker JR, Zambito G, Marella-Panth K, Lowik C, Kirkland TA, Mezzanotte L.
タイトル Evaluation of NanoLuc substrates for bioluminescence imaging of transferred cells in mice.
ジャーナル J Photochem Photobiol B
Abstract NanoLuc luciferase recently gained popularity due to its small size and superior bioluminescence performance. For in vivo imaging applications, NanoLuc has been limited by its substrate furimazine, which has low solubility and bioavailability. Herein, we compared the performances of recently reported NanoLuc luciferase substrates for in vivo imaging in mice. Two substrates with improved aqueous solubility, hydrofurimazine and fluorofurimazine, were evaluated along with three stabilized O-acetylated furimazine analogues, the hikarazines. All 5 analogues, when tested in vitro, displayed greater signal intensity and reaction duration, in comparison to the standard NanoLuc substrate, furimazine. The two best-performing analogues from the in vitro study were selected for further in vivo testing. The NanoLuc/fluorofurimazine pair demonstrated the highest bioluminescence intensity, post intravenous administration. It was found to be around 9-fold brighter compared to the NanoLuc/furimazine and 11-fold more intense than the NanoLuc/hikarazine-003 pair, with an average of 3-fold higher light emission when the substrate was injected intraperitoneally, in a subcutaneous model. Excitingly, despite the fact that NanoLuc/fluorofurimazine emits mostly blue light, we prove that cells trapped in mice lungs vasculature could be visualised via the NanoLuc/fluorofurimazine pair and compare the results to the AkaLuc/AkaLumine system. Therefore, among the tested analogues, fluorofurimazine enables higher substrate loading and improved optical imaging sensitivity in small animals, upgrading the use of NanoLuc derived bioluminescent systems for deep tissue imaging.
巻・号 216
ページ 112128
公開日 2021-3-1
DOI 10.1016/j.jphotobiol.2021.112128
PII S1011-1344(21)00006-3
PMID 33529963
MeSH Animals Furans / chemistry HEK293 Cells Humans Imidazoles / chemistry Lentivirus Infections Light Luciferases / chemistry* Luciferases / metabolism Luminescent Agents / chemistry* Luminescent Agents / metabolism Lung / diagnostic imaging* Male Mice, Inbred BALB C Mice, Nude Optical Imaging Pyrazines / chemistry Retinal Vessels / diagnostic imaging* Solubility Structure-Activity Relationship
リソース情報
遺伝子材料 pcDNA3 Venus-Akaluc (RDB15781)