論文 - 詳細
| RRC ID | 65483 |
|---|---|
| 著者 | Ito S, Masuda T, Noda M, Hu Q, Shimizu D, Kuroda Y, Eguchi H, Tobo T, Utsunomiya T, Mimori K. |
| タイトル | Prognostic Significance of PD-1, PD-L1 and CD8 Gene Expression Levels in Gastric Cancer. |
| ジャーナル | Oncology |
| Abstract |
INTRODUCTION:Anti-programmed cell death 1 (PD-1) therapies have shown promising clinical activity against gastric cancer (GC). We evaluated the clinical significance of immune-related gene expression in GC tissues to better understand the tumor immune microenvironment. METHODS:PD-1, PD-1 ligand 1 (PD-L1) and CD8 mRNA levels and clinicopathological factors, including survival, were examined by quantitative RT-PCR in 155 GC patients who underwent surgery. PD-1 and PD-L1 expression in tumor tissue from 24 GC patients was investigated by immunohistochemical analysis. RESULTS:PD-1, PD-L1 and CD8 mRNA levels were significantly lower in tumor tissue than in normal tissue (p < 0.0001, p < 0.05, and p < 0.0001). GC patients with low PD-1, PD-L1 and CD8 mRNA levels had significantly poorer overall survival (OS) than those with high PD-1, PD-L1 and CD8 mRNA levels, respectively (p < 0.001, p < 0.01 and p < 0.05). Low PD-1, PD-L1 and CD8 mRNA levels were more significantly associated with poor prognosis in undifferentiated-type GC patients than in differentiated-type GC patients (PD-1: differentiated p = 0.0071 vs. undifferentiated p = 0.0024; PD-L1: p = 0.6527 vs. p < 0.0001; CD8: p = 0.4465 vs. p < 0.05). Multivariate analysis showed that lymph node metastasis, peritoneal dissemination, distant metastasis, low PD-1 mRNA levels and low CD8 mRNA levels were independent prognostic factors for worse OS (low PD-1 mRNA level: OR 2.16, 95% CI 1.10-4.58, p < 0.05; low CD8 mRNA level: OR 2.55, 95% CI 1.12-6.90, p < 0.05). PD-1 and PD-L1 mRNA levels in immune cells were significantly associated with PD-1 and PD-L1 protein levels (both p < 0.05), respectively. CONCLUSIONS:PD-1, PD-L1 and CD8 mRNA levels may reflect antitumor immunity in GC, and low PD-1 and CD8 mRNA levels are potential predictive biomarkers for poor prognosis in GC patients who underwent surgery. |
| 巻・号 | 98(7) |
| ページ | 501-511 |
| 公開日 | 2020-1-1 |
| DOI | 10.1159/000506075 |
| PII | 000506075 |
| PMID | 32380498 |
| MeSH | Aged B7-H1 Antigen / genetics* B7-H1 Antigen / metabolism Biomarkers, Tumor / genetics CD8 Antigens / genetics* CD8-Positive T-Lymphocytes / immunology Female Gene Expression / immunology* Humans Lymphatic Metastasis / genetics Male Middle Aged Prognosis Programmed Cell Death 1 Receptor / genetics* Programmed Cell Death 1 Receptor / metabolism RNA, Messenger / genetics Real-Time Polymerase Chain Reaction Stomach Neoplasms / genetics* Tumor Microenvironment / immunology* |
| IF | 2.642 |
| リソース情報 | |
| ヒト・動物細胞 | TE-1(RCB1894) |