論文 - 詳細
| RRC ID | 65487 |
|---|---|
| 著者 | Tanaka S, Ono Y, Sakamoto K. |
| タイトル | DCEBIO facilitates myogenic differentiation via intermediate conductance Ca2+ activated K+ channel activation in C2C12 myoblasts. |
| ジャーナル | J Pharmacol Sci |
| Abstract |
Membrane hyperpolarization is suggested to be a trigger for skeletal muscle differentiation. We investigated whether DCEBIO, an opener of the small/intermediate conductance Ca2+ activated K+ (SKCa/IKCa) channels, increase myogenic differentiation in C2C12 skeletal myoblasts. DCEBIO significantly increased myotube formation, protein expression level of myosin heavy chain II, and mRNA expression level of myogenin in C2C12 myoblasts cultured in differentiation medium. DCEBIO induced myotube formation and hyperpolarization were reduced by the IKCa channel blocker TRAM-34, but not by the SKCa channel blocker apamin. These findings show that DCEBIO increases myogenic differentiation by activating IKCa channels. |
| 巻・号 | 133(4) |
| ページ | 276-279 |
| 公開日 | 2017-4-1 |
| DOI | 10.1016/j.jphs.2017.02.005 |
| PII | S1347-8613(17)30020-8 |
| PMID | 28302447 |
| MeSH | Apamin / pharmacology Benzimidazoles / pharmacology* Cell Differentiation / drug effects* Cells, Cultured Gene Expression / drug effects Humans Muscle Fibers, Skeletal / drug effects Muscle, Skeletal / cytology Myoblasts / cytology* Myoblasts / metabolism Myogenin / genetics Myogenin / metabolism Myosin Heavy Chains / genetics Myosin Heavy Chains / metabolism Potassium Channels, Calcium-Activated / antagonists & inhibitors Potassium Channels, Calcium-Activated / drug effects* Pyrazoles / pharmacology RNA, Messenger / metabolism |
| IF | 2.835 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | C2C12(RCB0987) |