| RRC ID |
65487
|
| 著者 |
Tanaka S, Ono Y, Sakamoto K.
|
| タイトル |
DCEBIO facilitates myogenic differentiation via intermediate conductance Ca2+ activated K+ channel activation in C2C12 myoblasts.
|
| ジャーナル |
J Pharmacol Sci
|
| Abstract |
Membrane hyperpolarization is suggested to be a trigger for skeletal muscle differentiation. We investigated whether DCEBIO, an opener of the small/intermediate conductance Ca2+ activated K+ (SKCa/IKCa) channels, increase myogenic differentiation in C2C12 skeletal myoblasts. DCEBIO significantly increased myotube formation, protein expression level of myosin heavy chain II, and mRNA expression level of myogenin in C2C12 myoblasts cultured in differentiation medium. DCEBIO induced myotube formation and hyperpolarization were reduced by the IKCa channel blocker TRAM-34, but not by the SKCa channel blocker apamin. These findings show that DCEBIO increases myogenic differentiation by activating IKCa channels.
|
| 巻・号 |
133(4)
|
| ページ |
276-279
|
| 公開日 |
2017-4-1
|
| DOI |
10.1016/j.jphs.2017.02.005
|
| PII |
S1347-8613(17)30020-8
|
| PMID |
28302447
|
| MeSH |
Apamin / pharmacology
Benzimidazoles / pharmacology*
Cell Differentiation / drug effects*
Cells, Cultured
Gene Expression / drug effects
Humans
Muscle Fibers, Skeletal / drug effects
Muscle, Skeletal / cytology
Myoblasts / cytology*
Myoblasts / metabolism
Myogenin / genetics
Myogenin / metabolism
Myosin Heavy Chains / genetics
Myosin Heavy Chains / metabolism
Potassium Channels, Calcium-Activated / antagonists & inhibitors
Potassium Channels, Calcium-Activated / drug effects*
Pyrazoles / pharmacology
RNA, Messenger / metabolism
|
| IF |
2.835
|
| リソース情報 |
| ヒト・動物細胞 |
C2C12(RCB0987) |
