RRC ID 65517
Author Endo H, Tanaka S, Imamura K, Adegawa S, Kikuta S, Sato R.
Title Cry toxin specificities of insect ABCC transporters closely related to lepidopteran ABCC2 transporters.
Journal Peptides
Abstract In this study, we examined insect and human ABCC transporters closely related to the lepidopteran ABC transporter C2 (ABCC2), a powerful receptor for the Bacillus thuringiensis Cry toxin, for their responses to various Cry toxins. ABCC2 and the lepidopteran ABC transporter C3 (ABCC3) conferred cultured cells with susceptibility to a lepidopteran-specific Cry1Aa toxin but not to lepidopteran-specific Cry1Ca and Cry1Da. One coleopteran ABCC transporter specifically responded to a coleopteran-specific Cry8Ca toxin. ABCC transporters from a dipteran insect and humans did not respond to any of the tested Cry toxins that are active to lepidopteran and coleopteran insects. These results yield important information for our understanding of insect specificity of Cry toxins and provide the first demonstration of a coleopteran ABCC transporter that serves as a Cry toxin receptor.
Volume 98
Pages 86-92
Published 2017-12-1
DOI 10.1016/j.peptides.2017.04.003
PII S0196-9781(17)30161-4
PMID 28416297
MeSH Amino Acid Sequence Animals Bacillus thuringiensis Toxins Bacterial Proteins / chemistry Bacterial Proteins / genetics Bacterial Proteins / metabolism* Bacterial Proteins / toxicity Coleoptera / drug effects Diptera / drug effects Endotoxins / chemistry Endotoxins / metabolism* Endotoxins / toxicity HEK293 Cells Hemolysin Proteins / chemistry Hemolysin Proteins / metabolism* Hemolysin Proteins / toxicity Humans Insect Proteins / chemistry Insect Proteins / genetics Insect Proteins / metabolism* Lepidoptera / drug effects* Multidrug Resistance-Associated Protein 2 Multidrug Resistance-Associated Proteins / chemistry Multidrug Resistance-Associated Proteins / genetics Multidrug Resistance-Associated Proteins / metabolism* Phylogeny Receptors, Cell Surface / chemistry Receptors, Cell Surface / genetics Receptors, Cell Surface / metabolism*
IF 2.843
Human and Animal Cells 293T(RCB2202)