RRC ID |
65517
|
著者 |
Endo H, Tanaka S, Imamura K, Adegawa S, Kikuta S, Sato R.
|
タイトル |
Cry toxin specificities of insect ABCC transporters closely related to lepidopteran ABCC2 transporters.
|
ジャーナル |
Peptides
|
Abstract |
In this study, we examined insect and human ABCC transporters closely related to the lepidopteran ABC transporter C2 (ABCC2), a powerful receptor for the Bacillus thuringiensis Cry toxin, for their responses to various Cry toxins. ABCC2 and the lepidopteran ABC transporter C3 (ABCC3) conferred cultured cells with susceptibility to a lepidopteran-specific Cry1Aa toxin but not to lepidopteran-specific Cry1Ca and Cry1Da. One coleopteran ABCC transporter specifically responded to a coleopteran-specific Cry8Ca toxin. ABCC transporters from a dipteran insect and humans did not respond to any of the tested Cry toxins that are active to lepidopteran and coleopteran insects. These results yield important information for our understanding of insect specificity of Cry toxins and provide the first demonstration of a coleopteran ABCC transporter that serves as a Cry toxin receptor.
|
巻・号 |
98
|
ページ |
86-92
|
公開日 |
2017-12-1
|
DOI |
10.1016/j.peptides.2017.04.003
|
PII |
S0196-9781(17)30161-4
|
PMID |
28416297
|
MeSH |
Amino Acid Sequence
Animals
Bacillus thuringiensis Toxins
Bacterial Proteins / chemistry
Bacterial Proteins / genetics
Bacterial Proteins / metabolism*
Bacterial Proteins / toxicity
Coleoptera / drug effects
Diptera / drug effects
Endotoxins / chemistry
Endotoxins / metabolism*
Endotoxins / toxicity
HEK293 Cells
Hemolysin Proteins / chemistry
Hemolysin Proteins / metabolism*
Hemolysin Proteins / toxicity
Humans
Insect Proteins / chemistry
Insect Proteins / genetics
Insect Proteins / metabolism*
Lepidoptera / drug effects*
Multidrug Resistance-Associated Protein 2
Multidrug Resistance-Associated Proteins / chemistry
Multidrug Resistance-Associated Proteins / genetics
Multidrug Resistance-Associated Proteins / metabolism*
Phylogeny
Receptors, Cell Surface / chemistry
Receptors, Cell Surface / genetics
Receptors, Cell Surface / metabolism*
|
IF |
2.843
|
リソース情報 |
ヒト・動物細胞 |
293T(RCB2202) |