RRC ID 65535
著者 Ichihara H, Kuwabara K, Matsumoto Y.
タイトル Trehalose Liposomes Suppress the Growth of Tumors on Human Lung Carcinoma-bearing Mice by Induction of Apoptosis In Vivo.
ジャーナル Anticancer Res
Abstract BACKGROUND/AIM:Previous evidence demonstrates that trehalose liposomes (DMTreC14) composed of L-α-dimyristoylphosphatidylcholine (DMPC) and α-D-glycopyranosyl-α-D-glucopyranoside monomyristate (TreC14) inhibit proliferation and invasion on lung carcinoma (A549 cells) in vitro. Here, we aimed to investigate suppressive effects of DMTreC14 on the growth of tumor on human lung carcinoma bearing mice.
MATERIALS AND METHODS:DMTreC14 composed of 30 mol% DMPC and 70 mol% TreC14 were prepared by the sonication method. Anti-tumor activities of DMTreC14 using the subcutaneous and orthotopic graft-bearing mice of A549 cells were investigated in vivo.
RESULTS:The remarkable reduction of volume and weight in subcutaneous tumors on subcutaneous lung carcinoma-bearing mice topically administrated with DMTreC14 were obtained. Apoptotic-positive cells in the subcutaneous tumor slice of subcutaneous lung carcinoma-bearing mice topically administrated with DMTreC14 were observed using TUNEL staining. Lung weights on the orthotopic graft-bearing mice of lung carcinoma intravenously administrated with DMTreC14 were markedly decreased compared to those of the control group. Remarkable decrease in dimensions of tumor area of lung on the orthotopic graft-bearing mice of lung carcinoma intravenously administrated with DMTreC14 was obtained in histological analysis using the hematoxylin and eosin staining.
CONCLUSION:Remarkably high anti-tumor activities of DMTreC14 for the subcutaneous and orthotopic graft-bearing mice of lung carcinoma accompanied with apoptosis were revealed for the first time in vivo.
巻・号 37(11)
ページ 6133-6139
公開日 2017-11-1
DOI 10.21873/anticanres.12062
PII 37/11/6133
PMID 29061794
MeSH Animals Apoptosis / drug effects* Cell Proliferation / drug effects Humans Liposomes / administration & dosage* Liposomes / chemistry Lung Neoplasms / drug therapy Lung Neoplasms / pathology* Mice Mice, Inbred BALB C Mice, Nude Trehalose / administration & dosage Trehalose / pharmacology* Tumor Cells, Cultured Xenograft Model Antitumor Assays
IF 1.994
リソース情報
ヒト・動物細胞 A549