| RRC ID |
65548
|
| 著者 |
Ogawa H, Wu X, Kawamoto K, Nishida N, Konno M, Koseki J, Matsui H, Noguchi K, Gotoh N, Yamamoto T, Miyata K, Nishiyama N, Nagano H, Yamamoto H, Obika S, Kataoka K, Doki Y, Mori M, Ishii H.
|
| タイトル |
MicroRNAs Induce Epigenetic Reprogramming and Suppress Malignant Phenotypes of Human Colon Cancer Cells.
|
| ジャーナル |
PLoS One
|
| Abstract |
Although cancer is a genetic disease, epigenetic alterations are involved in its initiation and progression. Previous studies have shown that reprogramming of colon cancer cells using Oct3/4, Sox2, Klf4, and cMyc reduces cancer malignancy. Therefore, cancer reprogramming may be a useful treatment for chemo- or radiotherapy-resistant cancer cells. It was also reported that the introduction of endogenous small-sized, non-coding ribonucleotides such as microRNA (miR) 302s and miR-369-3p or -5p resulted in the induction of cellular reprogramming. miRs are smaller than the genes of transcription factors, making them possibly suitable for use in clinical strategies. Therefore, we reprogrammed colon cancer cells using miR-302s and miR-369-3p or -5p. This resulted in inhibition of cell proliferation and invasion and the stimulation of the mesenchymal-to-epithelial transition phenotype in colon cancer cells. Importantly, the introduction of the ribonucleotides resulted in epigenetic reprogramming of DNA demethylation and histone modification events. Furthermore, in vivo administration of the ribonucleotides in mice elicited the induction of cancer cell apoptosis, which involves the mitochondrial Bcl2 protein family. The present study shows that the introduction of miR-302s and miR-369s could induce cellular reprogramming and modulate malignant phenotypes of human colorectal cancer, suggesting that the appropriate delivery of functional small-sized ribonucleotides may open a new avenue for therapy against human malignant tumors.
|
| 巻・号 |
10(5)
|
| ページ |
e0127119
|
| 公開日 |
2015-1-1
|
| DOI |
10.1371/journal.pone.0127119
|
| PII |
PONE-D-15-00929
|
| PMID |
25970424
|
| PMC |
PMC4430240
|
| MeSH |
Animals
Apoptosis
Cell Cycle
Cell Differentiation
Cell Line, Tumor
Cell Proliferation
Cellular Reprogramming
Colonic Neoplasms / genetics*
Colonic Neoplasms / pathology
Epigenesis, Genetic
Female
Gene Expression Regulation, Neoplastic
Humans
Kruppel-Like Factor 4
Mice, Inbred BALB C
Mice, Nude
MicroRNAs / genetics*
MicroRNAs / metabolism
Neoplasm Transplantation
Phenotype
|
| IF |
2.74
|
| リソース情報 |
| ヒト・動物細胞 |
HCT116(RCB2979)
CACO-2(RCB0988)
LoVo(RCB1639) |
