論文 - 詳細
| RRC ID | 65569 |
|---|---|
| 著者 | Matsubara H, Matsui Y, Miyata R, Nishimura Y, Yamamoto T, Tanaka T, Kondo M. |
| タイトル | New photic stimulating system with white light-emitting diodes to elicit electroretinograms from zebrafish larvae. |
| ジャーナル | Doc Ophthalmol |
| Abstract |
PURPOSE:The zebrafish is an established animal model commonly used in biological, neuroscience, and genetic research. We have developed a new light stimulating system using white light-emitting diodes (LEDs) to elicit ERGs from zebrafish larvae. The purpose of this study was to record full-field ERGs and to evaluate the inter-trial reliability of the ERGs recorded with our system from zebrafish larvae. METHODS:The stimulating device used white LEDs that were attached to a stereomicroscope, and the location of the recording electrode on the cornea could be monitored while the eye was being stimulated. Full-field scotopic and photopic ERGs were recorded from larvae at the age of 5-7 days post-fertilization (dpf). Intensity-response curves were constructed from the ERGs. Inter-trial reliability of the ERGs recorded by our system was evaluated. RESULTS:This stimulating system could be used for efficient and reliable ERG recordings from 5-7 dpf larvae. The amplitudes, implicit times, and the waveforms of the scotopic and photopic ERGs were similar to those reported in earlier studies. Inter-trial reliability of the amplitudes of the photopic ERG b-waves was excellent with an intra-class correlation coefficient of 0.98. CONCLUSION:We conclude that this new light stimulation system using white LEDs attached to a stereomicroscope will be helpful in recording reliable ERGs from zebrafish larvae. |
| 巻・号 | 135(2) |
| ページ | 147-154 |
| 公開日 | 2017-10-1 |
| DOI | 10.1007/s10633-017-9602-1 |
| PII | 10.1007/s10633-017-9602-1 |
| PMID | 28756596 |
| PMC | PMC5606953 |
| MeSH | Animals Color Vision Electroretinography / methods* Larva / physiology Night Vision Photic Stimulation / instrumentation* Reproducibility of Results Retina / physiology* Zebrafish / physiology* |
| リソース情報 | |
| ゼブラフィッシュ | RIKEN WT |