RRC ID 65576
Author Shitasako S, Ito Y, Ito R, Ueda Y, Shimizu Y, Ohshima T.
Title Wnt and Shh signals regulate neural stem cell proliferation and differentiation in the optic tectum of adult zebrafish.
Journal Dev Neurobiol
Abstract Adult neurogenesis occurs more commonly in teleosts, represented by zebrafish, than in mammals. Zebrafish is therefore considered a suitable model to study adult neurogenesis, for which the regulatory molecular mechanisms remain little known. Our previous study revealed that neuroepithelial-like neural stem cells (NSCs) are located at the edge of the dorsomedial region. We also showed that Notch signaling inhibits NSC proliferation in this region. In the present study, we reported the expression of Wnt and Shh signaling components in this region of the optic tectum. Moreover, inhibitors of Wnt and Shh signaling suppressed NSC proliferation, suggesting that these pathways promote NSC proliferation. Shh is particularly required for maintaining Sox2-positive NSCs. Our experimental data also indicate the involvement of these signaling pathways in neural differentiation from NSCs. © 2017 Wiley Periodicals, Inc. Develop Neurobiol 77: 1206-1220, 2017.
Volume 77(10)
Pages 1206-1220
Published 2017-10-1
DOI 10.1002/dneu.22509
PMID 28589698
MeSH Animals Animals, Genetically Modified Bromodeoxyuridine Cell Proliferation / drug effects Cell Proliferation / physiology* Central Nervous System Agents / pharmacology Hedgehog Proteins / antagonists & inhibitors Hedgehog Proteins / metabolism Imides / pharmacology Immunohistochemistry Membrane Proteins Microscopy, Confocal Microscopy, Fluorescence Neural Stem Cells / drug effects Neural Stem Cells / physiology* Neurogenesis / drug effects Neurogenesis / physiology* Patched-1 Receptor / metabolism Patched-2 Receptor / metabolism Proliferating Cell Nuclear Antigen / metabolism Quinolines / pharmacology SOX Transcription Factors / metabolism Superior Colliculi / drug effects Superior Colliculi / metabolism* Thiadiazoles / pharmacology Wnt Signaling Pathway / drug effects Wnt Signaling Pathway / genetics Wnt Signaling Pathway / physiology Zebrafish Zebrafish Proteins / antagonists & inhibitors Zebrafish Proteins / metabolism*
IF 3.935
Zebrafish RIKEN WT