論文 - 詳細
| RRC ID | 65628 |
|---|---|
| 著者 | Sarute N, Cheng H, Yan Z, Salas-Briceno K, Richner J, Rong L, Ross SR. |
| タイトル | Signal-regulatory protein alpha is an anti-viral entry factor targeting viruses using endocytic pathways. |
| ジャーナル | PLoS Pathog |
| Abstract |
Signal-regulatory protein alpha (SIRPA) is a well-known inhibitor of phagocytosis when it complexes with CD47 expressed on target cells. Here we show that SIRPA decreased in vitro infection by a number of pathogenic viruses, including New World and Old world arenaviruses, Zika virus, vesicular stomatitis virus and pseudoviruses bearing the Machupo virus, Ebola virus and SARS-CoV-2 glycoproteins, but not HSV-1, MLV or mNoV. Moreover, mice with targeted mutation of the Sirpa gene that renders it non-functional were more susceptible to infection with the New World arenaviruses Junín virus vaccine strain Candid 1 and Tacaribe virus, but not MLV or mNoV. All SIRPA-inhibited viruses have in common the requirement for trafficking to a low pH endosomal compartment. This was clearly demonstrated with SARS-CoV-2 pseudovirus, which was only inhibited by SIRPA in cells in which it required trafficking to the endosome. Similar to its role in phagocytosis inhibition, SIRPA decreased virus internalization but not binding to cell surface receptors. We also found that increasing SIRPA levels via treatment with IL-4 led to even greater anti-viral activity. These data suggest that enhancing SIRPA's activity could be a target for anti-viral therapies. |
| 巻・号 | 17(6) |
| ページ | e1009662 |
| 公開日 | 2021-6-1 |
| DOI | 10.1371/journal.ppat.1009662 |
| PII | PPATHOGENS-D-21-00311 |
| PMID | 34097709 |
| PMC | PMC8211255 |
| MeSH | Animals Antiviral Agents / pharmacology Cell Line Cell Membrane / virology Chlorocebus aethiops Drug Delivery Systems Endocytosis* Integrins / immunology Interleukin-4 / pharmacology Mice Mice, Knockout Protein Domains RNA Viruses / immunology* Receptors, Immunologic / genetics Receptors, Immunologic / physiology* Vero Cells Virus Internalization* |
| IF | 6.218 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 11 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC01544 |