論文 - 詳細
| RRC ID | 65661 |
|---|---|
| 著者 | Wei M, Li S, Yang Z, Cheng C, Li T, Le W. |
| タイトル | Tetrahedral DNA nanostructures functionalized by multivalent microRNA132 antisense oligonucleotides promote the differentiation of mouse embryonic stem cells into dopaminergic neurons. |
| ジャーナル | Nanomedicine |
| Abstract |
MicroRNA132 (miR132) negatively regulates the differentiation of mouse embryonic stem cells (ESCs) into dopaminergic (DAergic) neurons; in contrast, antisense oligonucleotide against miR132 (miR132-ASO) effectively blocks the activity of endogenous miR132 and thereafter promotes the differentiation of DAergic neurons. However, it is difficult for miR132-ASO to enter cells without a suitable delivery system. Tetrahedral DNA nanostructures (TDNs), as a new type of DNA-based nanocarrier, have great potential in biomedical applications and even have been reported to promote stem cell differentiation. In this study, we developed functional multivalent DNA nanostructures by appending miR132-ASO motifs to three-dimensional TDNs (miR132-ASO-TDNs). Our data clearly revealed that miR132-ASO-TDNs exposure can promote the differentiation of ESCs into DAergic neurons as well as elevate DA release from differentiated DAergic neurons. MiR132-ASO-TDNs could serve as a novel biofunctional nanomaterial to improve the efficiency of DAergic neurons differentiation. Our findings may also provide a new approach for stem cell therapy against neurodegenerative diseases. |
| 巻・号 | 34 |
| ページ | 102375 |
| 公開日 | 2021-6-1 |
| DOI | 10.1016/j.nano.2021.102375 |
| PII | S1549-9634(21)00018-6 |
| PMID | 33617970 |
| MeSH | Animals Cell Differentiation / drug effects* Cell Line DNA / chemistry* Dopaminergic Neurons / cytology Dopaminergic Neurons / drug effects* Mice MicroRNAs / genetics* Mouse Embryonic Stem Cells / cytology Mouse Embryonic Stem Cells / drug effects* Nucleic Acid Conformation* RNA, Antisense / pharmacology* |
| IF | 4.3 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-G2/PA6(RCB1127) |