Reference - Detail
| RRC ID | 65691 |
|---|---|
| Author | Arumugam S, Schmieder S, Pezeshkian W, Becken U, Wunder C, Chinnapen D, Ipsen JH, Kenworthy AK, Lencer W, Mayor S, Johannes L. |
| Title | Ceramide structure dictates glycosphingolipid nanodomain assembly and function. |
| Journal | Nat Commun |
| Abstract |
Gangliosides in the outer leaflet of the plasma membrane of eukaryotic cells are essential for many cellular functions and pathogenic interactions. How gangliosides are dynamically organized and how they respond to ligand binding is poorly understood. Using fluorescence anisotropy imaging of synthetic, fluorescently labeled GM1 gangliosides incorporated into the plasma membrane of living cells, we found that GM1 with a fully saturated C16:0 acyl chain, but not with unsaturated C16:1 acyl chain, is actively clustered into nanodomains, which depends on membrane cholesterol, phosphatidylserine and actin. The binding of cholera toxin B-subunit (CTxB) leads to enlarged membrane domains for both C16:0 and C16:1, owing to binding of multiple GM1 under a toxin, and clustering of CTxB. The structure of the ceramide acyl chain still affects these domains, as co-clustering with the glycosylphosphatidylinositol (GPI)-anchored protein CD59 occurs only when GM1 contains the fully saturated C16:0 acyl chain, and not C16:1. Thus, different ceramide species of GM1 gangliosides dictate their assembly into nanodomains and affect nanodomain structure and function, which likely underlies many endogenous cellular processes. |
| Volume | 12(1) |
| Pages | 3675 |
| Published | 2021-6-16 |
| DOI | 10.1038/s41467-021-23961-9 |
| PII | 10.1038/s41467-021-23961-9 |
| PMID | 34135326 |
| PMC | PMC8209009 |
| MeSH | Actins / chemistry CD59 Antigens / chemistry Cell Membrane / chemistry* Cell Membrane / drug effects Ceramides / chemistry* Cholera Toxin / chemistry Cholera Toxin / pharmacology Cholesterol / chemistry G(M1) Ganglioside / chemistry Glycosphingolipids / chemistry Glycosylphosphatidylinositols / chemistry Models, Biological Molecular Dynamics Simulation Phosphatidylserines / chemistry |
| IF | 12.121 |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 28 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | GM95(RCB1026) |