| RRC ID |
65693
|
| 著者 |
Kuwabara S, Tanimoto Y, Okutani M, Jie M, Haseda Y, Kinugasa-Katayama Y, Aoshi T.
|
| タイトル |
Microfluidics sorting enables the isolation of an intact cellular pair complex of CD8+ T cells and antigen-presenting cells in a cognate antigen recognition-dependent manner.
|
| ジャーナル |
PLoS One
|
| Abstract |
Adaptive immune responses begin with cognate antigen presentation-dependent specific interaction between T cells and antigen-presenting cells. However, there have been limited reports on the isolation and analysis of these cellular complexes of T cell-antigen-presenting cell (T/APC). In this study, we successfully isolated intact antigen-specific cellular complexes of CD8+ T/APC by utilizing a microfluidics cell sorter. Using ovalbumin (OVA) model antigen and OT-I-derived OVA-specific CD8+ T cells, we analyzed the formation of antigen-specific and antigen-non-specific T/APC cellular complexes and revealed that the antigen-specific T/APC cellular complex was highly stable than the non-specific one, and that the intact antigen-specific T/APC complex can be retrieved as well as enriched using a microfluidics sorter, but not a conventional cell sorter. The single T/APC cellular complex obtained can be further analyzed for the sequences of T cell receptor Vα and Vβ genes as well as cognate antigen information simultaneously. These results suggested that this approach can be applied for other antigen and CD8+ T cells of mice and possibly those of humans. We believe that this microfluidics sorting method of the T/APC complex will provide useful information for future T cell immunology research.
|
| 巻・号 |
16(6)
|
| ページ |
e0252666
|
| 公開日 |
2021-1-1
|
| DOI |
10.1371/journal.pone.0252666
|
| PII |
PONE-D-20-32262
|
| PMID |
34125844
|
| PMC |
PMC8202920
|
| MeSH |
Animals
Antigen Presentation / immunology*
Antigen-Presenting Cells / immunology*
Antigens / immunology*
CD8-Positive T-Lymphocytes / immunology*
CD8-Positive T-Lymphocytes / metabolism
Cell Communication / immunology
Cell Line, Tumor
Cell Separation / methods*
Flow Cytometry / methods
HEK293 Cells
Humans
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
Microfluidics / methods*
Receptors, Antigen, T-Cell / genetics
Receptors, Antigen, T-Cell / immunology
Receptors, Antigen, T-Cell / metabolism
Reproducibility of Results
|
| IF |
2.74
|
| リソース情報 |
| ヒト・動物細胞 |
293T(RCB2202) |
