論文 - 詳細
| RRC ID | 65693 |
|---|---|
| 著者 | Kuwabara S, Tanimoto Y, Okutani M, Jie M, Haseda Y, Kinugasa-Katayama Y, Aoshi T. |
| タイトル | Microfluidics sorting enables the isolation of an intact cellular pair complex of CD8+ T cells and antigen-presenting cells in a cognate antigen recognition-dependent manner. |
| ジャーナル | PLoS One |
| Abstract |
Adaptive immune responses begin with cognate antigen presentation-dependent specific interaction between T cells and antigen-presenting cells. However, there have been limited reports on the isolation and analysis of these cellular complexes of T cell-antigen-presenting cell (T/APC). In this study, we successfully isolated intact antigen-specific cellular complexes of CD8+ T/APC by utilizing a microfluidics cell sorter. Using ovalbumin (OVA) model antigen and OT-I-derived OVA-specific CD8+ T cells, we analyzed the formation of antigen-specific and antigen-non-specific T/APC cellular complexes and revealed that the antigen-specific T/APC cellular complex was highly stable than the non-specific one, and that the intact antigen-specific T/APC complex can be retrieved as well as enriched using a microfluidics sorter, but not a conventional cell sorter. The single T/APC cellular complex obtained can be further analyzed for the sequences of T cell receptor Vα and Vβ genes as well as cognate antigen information simultaneously. These results suggested that this approach can be applied for other antigen and CD8+ T cells of mice and possibly those of humans. We believe that this microfluidics sorting method of the T/APC complex will provide useful information for future T cell immunology research. |
| 巻・号 | 16(6) |
| ページ | e0252666 |
| 公開日 | 2021-1-1 |
| DOI | 10.1371/journal.pone.0252666 |
| PII | PONE-D-20-32262 |
| PMID | 34125844 |
| PMC | PMC8202920 |
| MeSH | Animals Antigen Presentation / immunology* Antigen-Presenting Cells / immunology* Antigens / immunology* CD8-Positive T-Lymphocytes / immunology* CD8-Positive T-Lymphocytes / metabolism Cell Communication / immunology Cell Line, Tumor Cell Separation / methods* Flow Cytometry / methods HEK293 Cells Humans Mice, Inbred C57BL Mice, Knockout Mice, Transgenic Microfluidics / methods* Receptors, Antigen, T-Cell / genetics Receptors, Antigen, T-Cell / immunology Receptors, Antigen, T-Cell / metabolism Reproducibility of Results |
| IF | 2.74 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 293T(RCB2202) |