| RRC ID |
65728
|
| 著者 |
Pangesty AI, Arahira T, Todo M.
|
| タイトル |
Development and characterization of hybrid tubular structure of PLCL porous scaffold with hMSCs/ECs cell sheet.
|
| ジャーナル |
J Mater Sci Mater Med
|
| Abstract |
Tissue engineering offers an alternate approach to providing vascular graft with potential to grow similar with native tissue by seeding autologous cells into biodegradable scaffold. In this study, we developed a combining technique by layering a sheet of cells onto a porous tubular scaffold. The cell sheet prepared from co-culturing human mesenchymal stem cells (hMSCs) and endothelial cells (ECs) were able to infiltrate through porous structure of the tubular poly (lactide-co-caprolactone) (PLCL) scaffold and further proliferated on luminal wall within a week of culture. Moreover, the co-culture cell sheet within the tubular scaffold has demonstrated a faster proliferation rate than the monoculture cell sheet composed of MSCs only. We also found that the co-culture cell sheet expressed a strong angiogenic marker, including vascular endothelial growth factor (VEGF) and its receptor (VEGFR), as compared with the monoculture cell sheet within 2 weeks of culture, indicating that the co-culture system could induce differentiation into endothelial cell lineage. This combined technique would provide cellularization and maturation of vascular construct in relatively short period with a strong expression of angiogenic properties.
|
| 巻・号 |
28(10)
|
| ページ |
165
|
| 公開日 |
2017-9-15
|
| DOI |
10.1007/s10856-017-5985-5
|
| PII |
10.1007/s10856-017-5985-5
|
| PMID |
28914404
|
| MeSH |
Bioprosthesis*
Blood Vessel Prosthesis
Cell Adhesion
Cell Differentiation
Cell Proliferation
Cell Shape
Cells, Cultured
Coculture Techniques
Endothelial Cells / cytology*
Humans
Materials Testing
Mesenchymal Stem Cells / cytology*
Polyesters / chemistry*
Porosity
Tissue Engineering / methods*
Tissue Scaffolds / chemistry*
|
| IF |
2.489
|
| リソース情報 |
| ヒト・動物細胞 |
UE6E7T-3(RCB2154) |
