| RRC ID |
65752
|
| 著者 |
Kondo T, Tsunematsu T, Yamada A, Arakaki R, Saito M, Otsuka K, Kujiraoka S, Ushio A, Kurosawa M, Kudo Y, Ishimaru N.
|
| タイトル |
Acceleration of tumor growth due to dysfunction in M1 macrophages and enhanced angiogenesis in an animal model of autoimmune disease.
|
| ジャーナル |
Lab Invest
|
| Abstract |
Both autoimmunity and tumor immunity are immune responses against self-tissues or cells. However, the precise similarity or difference between them remains unclear. In this study, to understand a novel mechanism of tumor immunity, we performed transplantation experiments with a murine autoimmune model, C57BL/6J (B6)/lpr mice. A melanoma cell line, B16F10 cells, or granulocyte macrophage colony-stimulating factor- overexpressing B16F10 (B16F10/mGM) cells were transplanted into B6 or B6/lpr mice. Tumor growth by transplanted B16F10/mGM cells was significantly accelerated in B6/lpr mice compared with that in B6 mice. The accumulation of M1 macrophages in the tumor tissues of B6/lpr recipient mice was significantly lower compared with that in the control mice. In vitro co-culture experiment showed that impaired differentiation into M1 macrophages was observed in B6/lpr mice. The number of tumor vessels and vascular endothelial growth factor (VEGF) expression were also significantly enhanced in the tumor tissues of B6/lpr mice compared with those in the B6 mice. Moreover, VEGF expression was correlated with the increased expression of hypoxia-inducible factor-1α in the tumor tissues of B6/lpr mice. These results suggest that dysfunctional tumor immunity and enhanced angiogenesis in autoimmunity influence tumor growth.
|
| 巻・号 |
96(4)
|
| ページ |
468-80
|
| 公開日 |
2016-4-1
|
| DOI |
10.1038/labinvest.2015.166
|
| PII |
labinvest2015166
|
| PMID |
26808709
|
| MeSH |
Animals
Autoimmune Diseases / genetics
Autoimmune Diseases / immunology
Autoimmune Diseases / metabolism
Cell Line, Tumor
Disease Models, Animal
Gene Expression / drug effects
Gene Expression / immunology
Hypoxia-Inducible Factor 1, alpha Subunit / genetics
Hypoxia-Inducible Factor 1, alpha Subunit / immunology
Hypoxia-Inducible Factor 1, alpha Subunit / metabolism
Immunohistochemistry
Interferon-gamma / genetics
Interferon-gamma / immunology
Interferon-gamma / metabolism
Macrophage Colony-Stimulating Factor / genetics
Macrophage Colony-Stimulating Factor / immunology
Macrophage Colony-Stimulating Factor / pharmacology
Macrophages / classification
Macrophages / immunology*
Melanoma, Experimental / blood supply
Melanoma, Experimental / genetics
Melanoma, Experimental / immunology*
Mice, Inbred C57BL
Mice, Inbred MRL lpr
Neovascularization, Pathologic / genetics
Neovascularization, Pathologic / immunology*
Neovascularization, Pathologic / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Tumor Burden / genetics
Tumor Burden / immunology*
Tumor Necrosis Factor-alpha / genetics
Tumor Necrosis Factor-alpha / immunology
Tumor Necrosis Factor-alpha / metabolism
Vascular Endothelial Growth Factor A / genetics
Vascular Endothelial Growth Factor A / immunology
Vascular Endothelial Growth Factor A / metabolism
|
| IF |
4.197
|
| リソース情報 |
| ヒト・動物細胞 |
B16F1(RCB2649)
B16F10(RCB2630)
B16F10/mGM(RCB1158) |
