論文 - 詳細
| RRC ID | 65752 |
|---|---|
| 著者 | Kondo T, Tsunematsu T, Yamada A, Arakaki R, Saito M, Otsuka K, Kujiraoka S, Ushio A, Kurosawa M, Kudo Y, Ishimaru N. |
| タイトル | Acceleration of tumor growth due to dysfunction in M1 macrophages and enhanced angiogenesis in an animal model of autoimmune disease. |
| ジャーナル | Lab Invest |
| Abstract |
Both autoimmunity and tumor immunity are immune responses against self-tissues or cells. However, the precise similarity or difference between them remains unclear. In this study, to understand a novel mechanism of tumor immunity, we performed transplantation experiments with a murine autoimmune model, C57BL/6J (B6)/lpr mice. A melanoma cell line, B16F10 cells, or granulocyte macrophage colony-stimulating factor- overexpressing B16F10 (B16F10/mGM) cells were transplanted into B6 or B6/lpr mice. Tumor growth by transplanted B16F10/mGM cells was significantly accelerated in B6/lpr mice compared with that in B6 mice. The accumulation of M1 macrophages in the tumor tissues of B6/lpr recipient mice was significantly lower compared with that in the control mice. In vitro co-culture experiment showed that impaired differentiation into M1 macrophages was observed in B6/lpr mice. The number of tumor vessels and vascular endothelial growth factor (VEGF) expression were also significantly enhanced in the tumor tissues of B6/lpr mice compared with those in the B6 mice. Moreover, VEGF expression was correlated with the increased expression of hypoxia-inducible factor-1α in the tumor tissues of B6/lpr mice. These results suggest that dysfunctional tumor immunity and enhanced angiogenesis in autoimmunity influence tumor growth. |
| 巻・号 | 96(4) |
| ページ | 468-80 |
| 公開日 | 2016-4-1 |
| DOI | 10.1038/labinvest.2015.166 |
| PII | labinvest2015166 |
| PMID | 26808709 |
| MeSH | Animals Autoimmune Diseases / genetics Autoimmune Diseases / immunology Autoimmune Diseases / metabolism Cell Line, Tumor Disease Models, Animal Gene Expression / drug effects Gene Expression / immunology Hypoxia-Inducible Factor 1, alpha Subunit / genetics Hypoxia-Inducible Factor 1, alpha Subunit / immunology Hypoxia-Inducible Factor 1, alpha Subunit / metabolism Immunohistochemistry Interferon-gamma / genetics Interferon-gamma / immunology Interferon-gamma / metabolism Macrophage Colony-Stimulating Factor / genetics Macrophage Colony-Stimulating Factor / immunology Macrophage Colony-Stimulating Factor / pharmacology Macrophages / classification Macrophages / immunology* Melanoma, Experimental / blood supply Melanoma, Experimental / genetics Melanoma, Experimental / immunology* Mice, Inbred C57BL Mice, Inbred MRL lpr Neovascularization, Pathologic / genetics Neovascularization, Pathologic / immunology* Neovascularization, Pathologic / metabolism Reverse Transcriptase Polymerase Chain Reaction Tumor Burden / genetics Tumor Burden / immunology* Tumor Necrosis Factor-alpha / genetics Tumor Necrosis Factor-alpha / immunology Tumor Necrosis Factor-alpha / metabolism Vascular Endothelial Growth Factor A / genetics Vascular Endothelial Growth Factor A / immunology Vascular Endothelial Growth Factor A / metabolism |
| IF | 4.197 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | B16F1(RCB2649) B16F10(RCB2630) B16F10/mGM(RCB1158) |