| RRC ID |
65763
|
| 著者 |
Kano D, Nakagami Y, Kurihara H, Hosokawa S, Zenda S, Kusumoto M, Fujii H, Kaneta T, Saito S, Uesawa Y, Kagaya H.
|
| タイトル |
Development of a double-stranded siRNA labelling method by using 99mTc and single photon emission computed tomography imaging.
|
| ジャーナル |
J Drug Target
|
| Abstract |
In vivo biodistribution of small interfering RNAs (siRNAs) is important to develop them for medical use. Therefore, novel single photon emitter-labelled siRNA was prepared by using diethylenetriamine-N,N,N',N″,N″-pentaacetic acid (DTPA) and poly(A) polymerase, and subsequently, real-time analysis of siRNA trafficking was performed by using single photon emission computed tomography (SPECT). This study aimed at assessing the use of 99mTc-radiolabelled siRNA targeting lacZ to detect lacZ expression in vivo. siRNA targeting lacZ was radiolabelled with 99mTc by using the bifunctional chelator DTPA, and the labelling efficiency and specific activity were determined. The probe stability in RNaseA was assessed. SPECT imaging was performed in mice overexpressing the lacZ gene in the liver. Radiolabelled siRNA remained highly stable in RNaseA solution at 37 °C. In SPECT imaging, significant 99mTc accumulation in the liver was observed in mice overexpressing the lacZ gene. 99mTc-labelled lacZ siRNA shows β-galactosidase-specific accumulation and appears promising for the visualisation of lacZ expression in vivo. Our labelled siRNA should be deliverable to specific regions overexpressing the target gene.
|
| 巻・号 |
25(2)
|
| ページ |
172-178
|
| 公開日 |
2017-2-1
|
| DOI |
10.1080/1061186X.2016.1223675
|
| PMID |
27588821
|
| MeSH |
Animals
Gamma Cameras
Gene Expression Regulation
Gene Transfer Techniques
HEK293 Cells
Humans
Lac Operon
Mice
Molecular Imaging
RNA, Double-Stranded*
RNA, Messenger / genetics
RNA, Messenger / metabolism*
RNA, Small Interfering*
Radionuclide Imaging*
Technetium / chemistry
Technetium / pharmacology*
Tomography, Emission-Computed, Single-Photon
|
| IF |
3.38
|
| リソース情報 |
| ヒト・動物細胞 |
293T(RCB2202) |
