論文 - 詳細
| RRC ID | 65778 |
|---|---|
| 著者 | Semlali A, Beji S, Ajala I, Rouabhia M. |
| タイトル | Effects of tetrahydrocannabinols on human oral cancer cell proliferation, apoptosis, autophagy, oxidative stress, and DNA damage. |
| ジャーナル | Arch Oral Biol |
| Abstract |
OBJECTIVE:Cannabinoids, including delta-8- and delta-9-tetrahydrocannabinol (THC) have a palliative care impact and may therefore be beneficial against cancer. The aim of this study was to investigate the effect of Δ9-THC and Δ8-THC on oral cancer cell behaviors. DESIGN:The Ca9-22 oral cancer cells were cultured in the presence or not of various concentrations of Δ9-THC and Δ8-THC for different times. The cultures were then used to measure cell viability/proliferation, apoptosis, autophagy, oxidative stress, antioxidant activity, and inhibition of signaling pathways MAP-Kinase, NF-κB, and β-catenin. RESULTS:Both cannabinoids were found to decrease cell viability/proliferation by blocking the cell cycle progression from the S to the G2/M phase and enhancing their apoptosis and autophagy. Δ9-THC and Δ8-THC also suppressed the migration/invasion by inhibiting epithelial-mesenchymal transition markers, such as E-cadherin, in addition to decreasing reactive oxygen species (ROS) production and increasing glutathione (GSH) and the expression of mtMP. Δ9-THC and Δ8-THC also downregulated cyclin D1, p53, NOXA, PUMAα, and DRAM expressions but increased p21 and H2AX expression. CONCLUSION:We demonstrated that cannabinoids (Δ9-THC and Δ8-THC) were able to decrease oral cancer cell growth through various mechanisms, including apoptosis, autophagy, and oxidative stress. These results suggest a potential use of these molecules as a therapy against oral cancer. |
| 巻・号 | 129 |
| ページ | 105200 |
| 公開日 | 2021-9-1 |
| DOI | 10.1016/j.archoralbio.2021.105200 |
| PII | S0003-9969(21)00163-1 |
| PMID | 34146926 |
| MeSH | Apoptosis Autophagy Cell Proliferation DNA Damage Dronabinol* / pharmacology Humans Mouth Neoplasms* / drug therapy Oxidative Stress |
| IF | 1.931 |
| リソース情報 | |
| ヒト・動物細胞 | Ca9-22(RCB1976) |