RRC ID 65781
著者 Soeta K, Yamaguchi R, Iuchi K, Hisatomi H, Yokoyama C.
タイトル Generation of Rat Monoclonal Antibody for Human IQGAP1 by Immunization of Three-Dimensional-Cultured Cancer Cells.
ジャーナル Monoclon Antib Immunodiagn Immunother
Abstract The scaffold protein IQ motif containing GTPase activating protein 1 (IQGAP1) is an adherens junction component in the epithelial tissue that binds many signaling and structural molecules to regulate biological processes. It is known that IQGAP1 is overexpressed in some tumors. In this study, we produced rat monoclonal antibodies (mAbs) through immunization of the lysate from three-dimensional (3D)-cultured DLD-1 cells to elucidate a characteristic feature of a tumor. In cancer research, 3D-cultured cancer cells are used as an intermediate model between in vitro cancer cell line cultures and in vivo tumors. Our results showed that mAb 7E11 recognized increasing antigen in the lysate of 3D-cultured cells comparing with two-dimensional-cultured cells, and its antigen is the human IQGAP1. Furthermore, we indicated that mAb 7E11 was used in immunoblotting, immunoprecipitation, and immunofluorescence staining. Therefore, it may be useful in the analysis of human cancer.
巻・号 40(3)
ページ 118-123
公開日 2021-6-1
DOI 10.1089/mab.2020.0046
PMID 34076498
MeSH Animals Antibodies, Monoclonal / immunology Antibodies, Monoclonal / pharmacology* Cell Culture Techniques, Three Dimensional Humans Immunization Neoplasms / immunology* Neoplasms / therapy Rats Signal Transduction / genetics ras GTPase-Activating Proteins / antagonists & inhibitors ras GTPase-Activating Proteins / immunology*
リソース情報
ヒト・動物細胞 Hep G2(RCB1886)